Contreras, A, Raxworthy, MJ, Wood, S et al. (2 more authors) (2019) Photodynamically Active Electrospun Fibers for Antibiotic-Free Infection Control. ACS Applied Bio Materials, 2 (10). pp. 4258-4270. ISSN 2576-6422
Abstract
Antimicrobial biomaterials are critical to aid in the regeneration of oral soft tissue and prevent or treat localized bacterial infections. With the rising trend in antibiotic resistance, there is a pressing clinical need for new antimicrobial chemistries and biomaterial design approaches enabling on-demand activation of antibiotic-free antimicrobial functionality following an infection that are environment-friendly, flexible and commercially viable. This study explores the feasibility of integrating a bioresorbable electrospun polymer scaffold with localized antimicrobial photodynamic therapy (aPDT) capability. To enable aPDT, we encapsulated a photosensitizer (PS) in polyester fibers in the PS inert state, so that the antibacterial function would be activated on-demand via a visible light source. Fibrous scaffolds were successfully electrospun from FDA-approved polyesters, either poly(ε-caprolactone (PCL) or poly[(rac-lactide)-co-glycolide] (PLGA), with encapsulated PS (either methylene blue (MB) or erythrosin B (ER)). These were prepared and characterized with regards to their loading efficiency (UV–vis spectroscopy), microarchitecture (SEM, porometry, and BET (Brunauer–Emmett–Teller) analysis), tensile properties, hydrolytic behavior (contact angle, dye release capability, degradability), and aPDT effect. The electrospun fibers achieved an ∼100 wt % loading efficiency of PS, which significantly increased their tensile modulus and reduced their average fiber diameter and pore size with respect to PS-free controls. In vitro, PS release varied between a burst release profile to limited release within 100 h, depending on the selected scaffold formulation, while PLGA scaffolds displayed significant macroscopic shrinkage and fiber merging, following incubation in phosphate buffered saline solution. Exposure of PS-encapsulated PCL fibers to visible light successfully led to at least a 1 log reduction in Escherichia coli viability after 60 min of light exposure, whereas PS-free electrospun controls did not inactive microbes. This study successfully demonstrates the significant potential of PS-encapsulated electrospun fibers as photodynamically active biomaterial for antibiotic-free infection control.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 American Chemical Society. This is an author produced version of a paper subsequently published in ACS Applied Bio Materials. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | fibers; photodynamic therapy; antimicrobial; erythrosin B; methylene blue; scaffold; bioresorbable; oral mucosa repair |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Mechanical Engineering (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Sep 2019 12:02 |
Last Modified: | 03 Sep 2020 00:38 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/acsabm.9b00543 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:150411 |