Shirgill, S., Nieves, D.J. orcid.org/0000-0002-0873-4418, Pike, J.A. orcid.org/0000-0003-4163-0335 et al. (15 more authors) (2025) Nano-org, a functional resource for single-molecule localisation microscopy data. Nature Communications, 16. 8674. ISSN: 2041-1723
Abstract
The nanoscale organisation of proteins plays a key role in diverse cellular processes, including signalling, adhesion, and structural integrity. Single-molecule localisation microscopy (SMLM) is a super-resolution imaging technique that captures the spatial distributions of proteins in cells with nanometre precision, enabling detailed studies of protein clustering and architecture. However, comparing such data across experiments remains challenging due to a lack of curated, functional resources. Here we present a publicly accessible, curated, and functional resource, termed “nano-org”, containing SMLM data representing the nanoscale distributions of proteins in cells. Nano-org is searchable by comparing the statistical similarity of the datasets it contains. This unique functionality allows the resource to be used to understand the relationships of nanoscale architectures between proteins, cell types or conditions, supporting the development of the field of spatial nano-omics.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2025. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
Keywords: | Nanoscale biophysics; Super-resolution microscopy |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Science (Sheffield) > School of Mathematical and Physical Sciences |
Date Deposited: | 06 Oct 2025 11:49 |
Last Modified: | 08 Oct 2025 08:46 |
Status: | Published |
Publisher: | Springer Science and Business Media LLC |
Refereed: | Yes |
Identification Number: | 10.1038/s41467-025-63674-x |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:232572 |