Nano-org, a functional resource for single-molecule localisation microscopy data

Abstract

The nanoscale organisation of proteins plays a key role in diverse cellular processes, including signalling, adhesion, and structural integrity. Single-molecule localisation microscopy (SMLM) is a super-resolution imaging technique that captures the spatial distributions of proteins in cells with nanometre precision, enabling detailed studies of protein clustering and architecture. However, comparing such data across experiments remains challenging due to a lack of curated, functional resources. Here we present a publicly accessible, curated, and functional resource, termed “nano-org”, containing SMLM data representing the nanoscale distributions of proteins in cells. Nano-org is searchable by comparing the statistical similarity of the datasets it contains. This unique functionality allows the resource to be used to understand the relationships of nanoscale architectures between proteins, cell types or conditions, supporting the development of the field of spatial nano-omics.

Metadata

Item Type:	Article
Authors/Creators:	Shirgill, S. Nieves, D.J. https://orcid.org/0000-0002-0873-4418 Pike, J.A. https://orcid.org/0000-0003-4163-0335 Ahmed, M.A. Abbott, H. Baragilly, M.H.H. https://orcid.org/0000-0003-4775-4742 Savoye, K. https://orcid.org/0009-0008-0111-8541 Worboys, J.D. https://orcid.org/0000-0002-8242-4901 Hazime, K.S. https://orcid.org/0000-0001-5125-2011 Bruggeman, E. Garcia, A. Williamson, D.J. https://orcid.org/0000-0002-6222-6297 Rubin-Delanchy, P. Peters, R. https://orcid.org/0000-0003-2946-7504 Davis, D.M. https://orcid.org/0000-0002-9182-291X Henriques, R. https://orcid.org/0000-0002-2043-5234 Lee, S.F. https://orcid.org/0000-0003-4492-5139 Owen, D.M. https://orcid.org/0000-0002-5284-2782
Copyright, Publisher and Additional Information:	© The Author(s) 2025. Open Access: This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Keywords:	Nanoscale biophysics; Super-resolution microscopy
Dates:	Accepted: 25 August 2025 Published (online): 30 September 2025 Published: 30 September 2025
Institution:	The University of Sheffield
Academic Units:	The University of Sheffield > Faculty of Science (Sheffield) > School of Mathematical and Physical Sciences
Date Deposited:	06 Oct 2025 11:49
Last Modified:	08 Oct 2025 08:46
Status:	Published
Publisher:	Springer Science and Business Media LLC
Refereed:	Yes
Identification Number:	10.1038/s41467-025-63674-x
Related URLs:	Dataset Dataset Dataset
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:232572