Farooque, S, Rose, PM, Benohoud, M et al. (2 more authors) (2018) Enhancing the potential exploitation of food waste: Extraction, purification, and characterization of renewable specialty chemicals from blackcurrants (Ribes nigrum L.). Journal of Agricultural and Food Chemistry, 66 (46). pp. 12265-12273. ISSN 0021-8561
Abstract
Natural colorants were extracted from renewable botanical sources, specifically waste epicarp from the blackcurrant fruit pressing industry. A process was developed which used acidified water extraction followed by a solid-phase extraction (SPE) purification stage which allowed the production of an anthocyanin-rich extract in good yields (ca. 2% w/w based on dry weight of raw material). The components in the extracts were extensively characterized by HPLC, mass spectrometry, IR, NMR and UV-Vis spectroscopy. HPLC confirmed presence of four anthocyanins: delphinidin-3-O-rutinoside (45%), cyanidin-3-O-rutinoside (31%) and the corresponding glucosides at 16% and 8%, respectively. On sequential liquid-liquid aqueous-organic partitioning of the post-SPE sample, monomeric anthocyanins (54.7%) and polymeric anthocyanins (18%) were found in the aqueous layer with 3-O-rutinosides of myricetin (3.1%) and quercetin (3.2%), whilst isopropylacetate achieved selective extraction of caffeic acid (3%), p-coumaric acid (5%), and myricetin (2.5%) and quercetin (3.2%) aglycons. 3-O-Glucosides of myricetin (3.1%) and quercetin (2%), along with nigrumin-p-coumarate (1%) and nigrumin ferulate (0.5%) were selectively extracted from the remaining aqueous fraction using ethylacetate. This allowed for near total quantification of the blackcurrant extract composition.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | Copyright © 2018 American Chemical Society. This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Journal of Agricultural and Food Chemistry after peer review. To access the final edited and published work see https://doi.org/10.1021/acs.jafc.8b04373 |
Keywords: | Anthocyanin; polyphenol; fruit waste; dyes; quantification; characterization |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) |
Funding Information: | Funder Grant number Technology Strategy Board BW004A Technology Strategy Board BW004A DEFRA Dept for Env. Food & Rural Affairs CSA 6527 LK0821 KERACOL Innovate UK fka Technology Strategy Board (TSB) BW004A/130229 |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Oct 2018 14:19 |
Last Modified: | 09 Nov 2019 01:38 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/acs.jafc.8b04373 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:137865 |