Gala De Pablo, J orcid.org/0000-0003-0557-9632, Armistead, FJ orcid.org/0000-0003-1274-5379, Peyman, SA orcid.org/0000-0002-1600-5100 et al. (4 more authors) (2018) Biochemical fingerprint of colorectal cancer cell lines using label-free live single-cell Raman spectroscopy. Journal of Raman Spectroscopy, 49 (8). pp. 1323-1332. ISSN 0377-0486
Abstract
Label-free live single-cell Raman spectroscopy was used to obtain a chemical fingerprint of colorectal cancer cells including the classification of the SW480 and SW620 cell line model system, derived from primary and secondary tumour cells from the same patient. High-quality Raman spectra were acquired from hundreds of live cells, showing high reproducibility between experiments. Principal component analysis with linear discriminant analysis yielded the best cell classification, with an accuracy of 98.7±0.3% (standard error) when compared to discrimination trees or support vector machines. SW480 showed higher content of the disordered secondary protein structure amide III band, whereas SW620 showed larger α-helix and β-sheet band content. The SW620 cell line also displayed higher nucleic acid, phosphates, saccharide, and CH₂ content. HL60, HT29, HCT116, SW620 and SW480 live single-cell spectra were classified using PCA/LDA with an accuracy of 92.4±0.4% (standard error), showing differences mainly in the β-sheet content, the cytochrome C bands, the CH-stretching regions, the lactate contributions and the DNA content. The lipids contributions above 2900 cm¯¹ and the lactate contributions at 1785 cm¯¹ appeared to be dependent on the colorectal adenocarcinoma stage, the advanced stage cell lines showing lower lipid and higher lactate content. The results demonstrate that these cell lines can be distinguished with high confidence, suggesting that Raman spectroscopy on live cells can distinguish between different disease stages, and could play an important role clinically as a diagnostic tool for cell phenotyping.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Editors: |
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Copyright, Publisher and Additional Information: | © 2018 The Authors. Journal of Raman Spectroscopy Published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Raman spectroscopy; single-cell; living-cells; metastasis; colorectal cancer |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Inst of Biomed & Clin Sciences (LIBACS) (Leeds) > Genetics (LIBACS) (Leeds) |
Funding Information: | Funder Grant number NIHR National Inst Health Research Not Known |
Depositing User: | Symplectic Publications |
Date Deposited: | 06 Apr 2018 15:20 |
Last Modified: | 16 Dec 2024 14:50 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/jrs.5389 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:129302 |
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