Karpinska, B, Zhang, K, Rasool, B et al. (6 more authors) (2018) The redox state of the apoplast influences the acclimation of photosynthesis and leaf metabolism to changing irradiance. Plant, Cell and Environment, 41 (5). pp. 1083-1097. ISSN 0140-7791
Abstract
The redox state of the apoplast is largely determined by ascorbate oxidase (AO) activity. The influence of AO activity on leaf acclimation to changing irradiance was explored in wild‐type (WT) and transgenic tobacco (Nicotiana tobaccum) lines containing either high [pumpkin AO (PAO)] or low [tobacco AO (TAO)] AO activity at low [low light (LL); 250 μmol m⁻² s⁻¹] and high [high light (HL); 1600 μmol m⁻² s⁻¹] irradiance and following the transition from HL to LL. AO activities changed over the photoperiod, particularly in the PAO plants. AO activity had little effect on leaf ascorbate, which was significantly higher under HL than under LL. Apoplastic ascorbate/dehydroascorbate (DHA) ratios and threonate levels were modified by AO activity. Despite decreased levels of transcripts encoding ascorbate synthesis enzymes, leaf ascorbate increased over the first photoperiod following the transition from HL to LL, to much higher levels than LL‐grown plants. Photosynthesis rates were significantly higher in the TAO leaves than in WT or PAO plants grown under HL but not under LL. Sub‐sets of amino acids and fatty acids were lower in TAO and WT leaves than in the PAO plants under HL, and following the transition to LL. Light acclimation processes are therefore influenced by the apoplastic as well as chloroplastic redox state.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
Keywords: | ascorbate; light acclimation; photosynthesis; redox regulation; transcriptome |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Funding Information: | Funder Grant number BBSRC BB/M009130/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 24 Mar 2017 12:41 |
Last Modified: | 23 Jun 2023 22:26 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/pce.12960 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:114084 |
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Filename: Karpinska_et_al-2018-Plant,_Cell_&_Environment.pdf
Licence: CC-BY 4.0
Filename: Table S1 Transcript abundance end of HL day 7.xlsx
Description: Spreadsheet
Licence: CC-BY 4.0
Filename: Table S2 Transcript abundance following 24h LL day 8.xlsx
Description: Spreadsheet
Licence: CC-BY 4.0