Versteegh, Emma, Black, Stuart and Hodson, Mark Edward orcid.org/0000-0002-8166-1526 (2017) Carbon isotope fractionation between amorphous calcium carbonate and calcite in earthworm-produced calcium carbonate. Applied Geochemistry. pp. 1-31. ISSN 0883-2927
Abstract
In this study we investigate carbon isotope fractionation during the crystallization of biogenic calcium carbonate. Several species of earthworm including Lumbricus terrestris secrete CaCO3. Initially a milky fluid comprising micro-spherules of amorphous CaCO3 (ACC) is secreted into pouches of the earthworm calciferous gland. The micro-spherules coalesce and crystalize to form millimetre scale granules, largely comprising calcite. These are secreted into the earthworm intestine and from there into the soil. L. terrestris were cultured for 28 days in two different soils, moistened with three different mineral waters at 10, 16 and 20 °C. The milky fluid in the calciferous glands, granules in the pouches of the calciferous glands and granules excreted into the soil were collected and analysed by FTIR spectroscopy to determine the form of CaCO3 present and by IRMS to determine δ13C values. The milky fluid was ACC. Granules removed from the pouches and soil were largely calcite; the granules removed from the pouches contained more residual ACC than those recovered from the soil. The δ13C values of milky fluid and pouch granules became significantly more negative with increasing temperature (p < 0.001). For samples from each temperature treatment, δ13C values became significantly (p < 0.001) more negative from the milky fluid to the pouch granules to the soil granules (-13.77, -14.69 and -15.00 respectively at 10 °C; -14.37, -15.07 and -15.18 respectively at 16 °C and -14.89, -15.41 and -15.65 respectively at 20 °C). Fractionation of C isotopes occurred as the ACC recrystallized to form calcite with the fractionation factor εcalcite-ACC = -1.20 ± 0.52 %0. This is consistent with the crystallization involving dissolution and reprecipitation rather than a solid state rearrangement. Although C isotopic fractionation has previously been described between different species of dissolved inorganic carbon and various CaCO3 polymorphs, this is the first documented evidence for C isotope fractionation between ACC and the calcite it recrystallizes to. This phenomenon may prove important for the interpretation of CaCO3-based C isotope environmental proxies.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2017 The Authors. Published by Elsevier Ltd. |
Keywords: | calcite,carbon isotopes,fractionation,earthworm,calcium carboante,amorphous calcium carbonate |
Dates: |
|
Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Environment and Geography (York) |
Funding Information: | Funder Grant number NATURAL ENVIRONMENT RESEARCH COUNCIL NE/H021914/2 |
Depositing User: | Pure (York) |
Date Deposited: | 31 Jan 2017 14:37 |
Last Modified: | 02 Apr 2025 23:09 |
Published Version: | https://doi.org/10.1016/j.apgeochem.2017.01.017 |
Status: | Published online |
Refereed: | Yes |
Identification Number: | 10.1016/j.apgeochem.2017.01.017 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:111472 |
Downloads
Filename: Versteegh_et_al_revised_final.docx
Description: Versteegh et al revised final
Licence: CC-BY-NC-ND 2.5
Filename: 1_s2.0_S0883292716304747_main.pdf
Description: 1-s2.0-S0883292716304747-main
Licence: CC-BY-NC-ND 2.5
Filename: 1_s20_S0883292716304747_main.pdf
Description: 1-s20-S0883292716304747-main
Licence: CC-BY 2.5