Darling, A.L., Middleton, B.A., Gossiel, F. et al. (3 more authors) (2025) Demonstration of an intrinsic circadian rhythm in bone resorption. Scientific Reports, 15 (1). 32558. ISSN: 2045-2322
Abstract
Daily 24 h rhythms in bone turnover have been demonstrated but whether these rhythms are intrinsically generated circadian rhythms is not known. We thus aimed to investigate this using the commonly used constant routine protocol where external factors such as meals, activity, sleep/wake and light/dark are kept constant. Serum procollagen type I N-terminal propeptide (sPINP) (marker of bone formation) and C-terminal telopeptide of type 1 collagen (sCTX) (marker of bone resorption), were measured in 2 hourly blood samples taken sequentially across 26 h in healthy individuals (n = 22, aged 19–33 years, 50% female). Concentration of sCTX showed a cosine rhythm in all males (acrophase (peak) time (mean ± SEM) 02:48 ± 14 h:min, amplitude 0.15 ± 0.02 ng/mL). All of the females had a statistically significant cosine + linear fit (acrophase 03:24 ± 20 h:min, amplitude 0.05 ± 0.01 ng/mL). There was no sex difference in acrophase, but females had a significantly smaller amplitude (P < 0.001). For sP1NP, only 4 males (36%) and 1 female showed statistically significant rhythms for either cosine, or cosine + linear models. Overall, sCTX, but not sPINP, exhibited a robust circadian rhythm in both males and females. This finding suggests that the circadian clock regulation of bone resorption by osteoclasts is robust, whereas circadian clock regulation of bone formation by osteoblasts is minimal.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s) 2025. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
Keywords: | Bone turnover; Circadian; Collagen type 1 C-telopeptide; Type 1 procollagen N-terminal peptide; Constant routine study |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > School of Medicine and Population Health |
Date Deposited: | 29 Sep 2025 13:49 |
Last Modified: | 29 Sep 2025 13:49 |
Status: | Published |
Publisher: | Springer Science and Business Media LLC |
Refereed: | Yes |
Identification Number: | 10.1038/s41598-025-16722-x |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:232331 |