Marino, S., Bishop, R.T., de Ridder, D. et al. (2 more authors) (2019) 2D and 3D in vitro co-culture for cancer and bone cell interaction studies. In: Idris, A.I., (ed.) Bone Research Protocols. Methods in Molecular Biology, MIMB 1914. Humana New York, NY, pp. 71-98. ISBN: 9781493989966. ISSN: 1064-3745. EISSN: 1940-6029.
Abstract
Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression.
Metadata
| Item Type: | Book Section |
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| Authors/Creators: |
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| Editors: |
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| Copyright, Publisher and Additional Information: | © 2019 Springer Science+Business Media, LLC, part of Springer Nature. |
| Keywords: | Cancer cells; In vitro co-culture; Osteoblasts; Osteoclasts; Osteocytes; Adipocytes; Animals; Bombyx; Bone Marrow Cells; Cell Communication; Cell Differentiation; Cell Line, Tumor; Coculture Techniques; Humans; Mice; Osteoclasts; Osteocytes; Primary Cell Culture; Stromal Cells; Tissue Engineering; Tissue Scaffolds |
| Dates: |
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| Institution: | The University of Sheffield |
| Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Oncology (Sheffield) |
| Depositing User: | Symplectic Sheffield |
| Date Deposited: | 04 Sep 2025 13:20 |
| Last Modified: | 04 Sep 2025 13:20 |
| Status: | Published |
| Publisher: | Humana New York, NY |
| Series Name: | Methods in Molecular Biology |
| Refereed: | Yes |
| Identification Number: | 10.1007/978-1-4939-8997-3_5 |
| Related URLs: | |
| Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:230850 |
CORE (COnnecting REpositories)
CORE (COnnecting REpositories)