Whale, T.F. orcid.org/0000-0002-1062-2685 (2023) Quantification of the Ice Nucleation Activity of Ice-Binding Proteins Using a Microliter Droplet Freezing Experiment. In: Drori, R. and Stevens, C., (eds.) Ice Binding Proteins: Methods and Protocols. Methods in Molecular Biology, 2730 . Springer Nature , pp. 121-134. ISBN 978-1-0716-3502-5
Abstract
Many ice-binding proteins can stimulate nucleation of ice from supercooled liquid water. Quantification of ice nucleation usually involves cooling a population of droplets and observing their freezing temperatures. Here, a method for measuring the freezing temperatures of microliter-scale droplets is described and its application to quantifying a substances’ ice nucleation activity discussed.
Metadata
| Item Type: | Book Section |
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| Authors/Creators: |
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| Editors: |
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| Keywords: | Ice nucleation, Droplet freezing |
| Dates: |
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| Institution: | The University of Leeds |
| Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Earth and Environment (Leeds) |
| Depositing User: | Symplectic Publications |
| Date Deposited: | 18 Jul 2025 09:22 |
| Last Modified: | 18 Jul 2025 09:22 |
| Status: | Published |
| Publisher: | Springer Nature |
| Series Name: | Methods in Molecular Biology |
| Identification Number: | 10.1007/978-1-0716-3503-2_9 |
| Related URLs: | |
| Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:229302 |

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