Rausch, M. orcid.org/0000-0002-8485-9220, Iqbal, N. orcid.org/0000-0002-2801-707X, Pathak, S. et al. (2 more authors) (Cover date: September 2023) Organoid Models and Next-Generation Sequencing for Bone Marrow and Related Disorders. Organoids, 2 (3). pp. 123-139. ISSN 2674-1172
Abstract
Challenges to the musculoskeletal system negatively impact the quality of life of people suffering from them, leading to pain, a decline in mobility, genetic alterations, and potential disorders. The bone marrow (BM) forms an integral part of the musculoskeletal system responsible for erythropoiesis and optimal survival of the various immune and stem cells within the BM. However, due to its dynamic and complex three-dimensional (3D) structure, replicating the BM physiologically in traditional two-dimensional (2D) cell culture settings is often challenging, giving rise to the need for 3D in vitro models to better dissect the BM and its regeneration. Several researchers globally have been investigating various approaches to define an appropriate 3D model for their research. Organoids are novel preclinical models that provide a 3D platform for several tissues and have been analysed using next-generation sequencing (NGS) to identify new molecular pathways at the genetic level. The 3D in vitro models and organoids are increasingly considered important platforms for precision medicine. This review outlines the current knowledge of organoid and 3D in vitro models for the BM. We also discuss different types of 3D models which may be more adaptable for the BM. Finally, we critically review the NGS techniques used for such models and the future combination of these techniques.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). |
Keywords: | bone marrow; organoids; in vitro; 3D modelling; bone regeneration; genetics; next-generation sequencing |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Institute of Rheumatology & Musculoskeletal Medicine (LIRMM) (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 08 Aug 2023 14:22 |
Last Modified: | 08 Aug 2023 14:26 |
Published Version: | https://www.mdpi.com/2674-1172/2/3/10 |
Status: | Published |
Publisher: | MDPI |
Identification Number: | 10.3390/organoids2030010 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:202212 |
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