Borland, S.J. orcid.org/0000-0001-7358-5829, Behnsen, J. orcid.org/0000-0003-1511-3051, Ashton, N. orcid.org/0000-0003-3366-1617 et al. (5 more authors) (2020) X-ray micro-computed tomography: an emerging technology to analyze vascular calcification in animal models. International Journal of Molecular Sciences, 21 (12). 4538. ISSN 1661-6596
Abstract
Vascular calcification describes the formation of mineralized tissue within the blood vessel wall, and it is highly associated with increased cardiovascular morbidity and mortality in patients with chronic kidney disease, diabetes, and atherosclerosis. In this article, we briefly review different rodent models used to study vascular calcification in vivo, and critically assess the strengths and weaknesses of the current techniques used to analyze and quantify calcification in these models, namely 2-D histology and the o-cresolphthalein assay. In light of this, we examine X-ray micro-computed tomography (µCT) as an emerging complementary tool for the analysis of vascular calcification in animal models. We demonstrate that this non-destructive technique allows us to simultaneously quantify and localize calcification in an intact vessel in 3-D, and we consider recent advances in µCT sample preparation techniques. This review also discusses the potential to combine 3-D µCT analyses with subsequent 2-D histological, immunohistochemical, and proteomic approaches in correlative microscopy workflows to obtain rich, multifaceted information on calcification volume, calcification load, and signaling mechanisms from within the same arterial segment. In conclusion we briefly discuss the potential use of µCT to visualize and measure vascular calcification in vivo in real-time.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | correlative microscopy; histology; micro-CT; mouse models; vascular calcification; Animals; Atherosclerosis; Humans; Imaging, Three-Dimensional; Microscopy; Models, Animal; Proteomics; Renal Insufficiency, Chronic; Vascular Calcification; X-Ray Microtomography |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Medicine, Dentistry and Health (Sheffield) > Department of Infection, Immunity and Cardiovascular Disease |
Funding Information: | Funder Grant number Engineering and Physical Sciences Research Council EP/R00661X/1 |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 01 Aug 2023 15:40 |
Last Modified: | 01 Aug 2023 15:40 |
Published Version: | http://dx.doi.org/10.3390/ijms21124538 |
Status: | Published |
Publisher: | MDPI AG |
Refereed: | Yes |
Identification Number: | 10.3390/ijms21124538 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:202067 |