1000 spider silkomes: linking sequences to silk physical properties

Abstract

Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, and sustainable biopolymers. However, the entirety of their diversity still remains elusive, and silks that exceed the performance limits of industrial fibers are constantly being found. We obtained transcriptome assemblies from 1098 species of spiders to comprehensively catalog silk gene sequences and measured the mechanical, thermal, structural, and hydration properties of the dragline silks of 446 species. The combination of these silk protein genotype-phenotype data revealed essential contributions of multicomponent structures with major ampullate spidroin 1 to 3 paralogs in high-performance dragline silks and numerous amino acid motifs contributing to each of the measured properties. We hope that our global sampling, comprehensive testing, integrated analysis, and open data will provide a solid starting point for future biomaterial designs.

Metadata

Item Type:	Article
Authors/Creators:	Arakawa, K. Kono, N. Malay, A.D. Tateishi, A. Ifuku, N. Masunaga, H. Sato, R. Tsuchiya, K. Ohtoshi, R. Pedrazzoli, D. Shinohara, A. Ito, Y. Nakamura, H. Tanikawa, A. Suzuki, Y. Ichikawa, T. Fujita, S. Fujiwara, M. Tomita, M. Blamires, S.J. Chuah, J.-A. Craig, H. Foong, C.P. Greco, G. Guan, J. Holland, C. https://orcid.org/0000-0003-0913-2221 Kaplan, D.L. Sudesh, K. Mandal, B.B. Norma-Rashid, Y. Oktaviani, N.A. Preda, R.C. Pugno, N.M. Rajkhowa, R. Wang, X. Yazawa, K. Zheng, Z. Numata, K.
Copyright, Publisher and Additional Information:	Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
Dates:	Published: 12 October 2022 Published (online): 12 November 2022 Accepted: 19 August 2022
Institution:	The University of Sheffield
Academic Units:	The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield)
Depositing User:	Symplectic Sheffield
Date Deposited:	03 Nov 2022 14:33
Last Modified:	03 Nov 2022 14:33
Status:	Published
Publisher:	American Association for the Advancement of Science (AAAS)
Refereed:	Yes
Identification Number:	10.1126/sciadv.abo6043
Related URLs:	PubMed URL
Open Archives Initiative ID (OAI ID):	oai:eprints.whiterose.ac.uk:192952

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1000 spider silkomes: linking sequences to silk physical properties

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