Sánchez‐Velázquez, OA, Ribéreau, S, Mondor, M et al. (3 more authors) (Cover date: June 2021) Impact of processing on the in vitro protein quality, bioactive compounds, and antioxidant potential of 10 selected pulses. Legume Science, 3 (2). e88. ISSN 2639-6181
Abstract
Pulses are consumed worldwide with different processing methods, which may impact their digestibility, protein quality, and composition. This study aims to analyze the effect of extrusion, baking, and cooking on protein nutritional parameters; bioactive compounds; and the impact on antioxidant capacity (AOX) of 10 selected pulses. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that thermal processing causes modifications to the main storage proteins in pulses. Heating decreased saponin content from 12% to 44% in most heat-processed samples; phytates were reduced 30%–84%, and polyphenol content decreased 28%–66%. In addition, the in vitro protein digestibility (IVPD) was enhanced 2.5%–9.5%, 3.5%–10.7%, and 2.2%–8.4% by extrusion, cooking, and baking, respectively. AOX showed an improvement in all processed samples (compared to raw flour) evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and by the oxygen radical absorbance capacity (ORAC). Fe²⁺ chelation showed that extruded and baked chickpea exhibited a decrease in IC50 by 40% and 70%, respectively. Extruded green and yellow split pea presented the highest Fe²⁺ chelation, improving by 11%–17% and 13–80%, respectively, when compared to the raw samples. Reducing power was enhanced by 26% in extruded chickpea, 18% and 29% in extruded and baked faba bean, respectively, and 50% in baked navy bean, when compared to the raw samples. Extrusion showed the highest β-carotene AOX improvements (IC₅₀ 90%–96%). In this study, it was demonstrated that pulses AOX attributes can be enhanced by thermal processing; however, this will depend on the legume species and heating process applied. Furthermore, cooking seems to be the most effective thermal method to decrease saponins and phenolics, while extrusion reduced effectively phytic acid on bean samples, and cooking for the rest of pulses. All heating treatments affected positively IVPD, while the highest in vitro protein-digestibility corrected amino acid score (IVPDCAAS) values were observed for baked pulses. Employing adequate processing methods represents an effective strategy to improve the digestibility of their proteins, as well as increasing the antioxidant potential of the resulting ingredients.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 The Authors. Legume Science published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | antioxidant activity; bioactive compounds; in vitro protein digestibility; IVPDCAAS; nutritional parameters; processed pulses |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Nutrition and Public Health (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 30 Mar 2021 15:31 |
Last Modified: | 25 Jun 2023 22:37 |
Status: | Published |
Publisher: | Wiley Open Access |
Identification Number: | 10.1002/leg3.88 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:172671 |