Near infrared spectroscopy of superficial and deep rectus femoris reveals markedly different exercise response to superficial vastus lateralis

To date our knowledge of skeletal muscle deoxygenation as measured by near-infrared spectroscopy (NIRS) is predicated almost exclusively on sampling of superficial muscle(s), most commonly the vastus lateralis (VL-s). Recently developed high power NIRS facilitates simultaneous sampling of deep (i.e., rectus femoris, RF-d) and superficial muscles of RF (RF-s) and VL-s. Because deeper muscle is more oxidative with greater capillarity and sustains higher blood flows than superficial muscle, we used time-resolved NIRS to test the hypotheses that, following exercise onset, the RF-d has slower deoxy[Hb+Mb] kinetics with reduced amplitude than superficial muscles. Thirteen participants performed cycle exercise transitions from unloaded to heavy work rates. Within the same muscle (RF-s vs. RF-d) deoxy[Hb+Mb] kinetics (mean response time, MRT) and amplitudes were not different. However, compared with the kinetics of VL-s, deoxy[Hb+Mb] of RF-s and RF-d were slower (MRT: RF-s, 51 ± 23; RF-d, 55 ± 29; VL-s, 18 ± 6 s; P<0.05). Moreover, the amplitude of total[Hb+Mb] was greater for VL-s than both RF-s and RF-d (P<0.05). Whereas pulmonary VO₂ kinetics (i.e., on versus off) were symmetrical in heavy exercise, there was a marked on-off asymmetry of deoxy[Hb+Mb] for all three sites i.e., MRT-off > MRT-on (P<0.05). Collectively these data reveal profoundly different O₂ transport strategies, with the RF-s and RF-d relying proportionately more on elevated perfusive and the VL-s on diffusive O₂ transport. These disparate O₂ transport strategies and their temporal profiles across muscles have previously been concealed within the “global” pulmonary VO₂ response.