Bell, SM orcid.org/0000-0002-3022-9864, Kelly, SA, Hoyle, JA et al. (5 more authors) (1991) c-Ki-ras gene mutations in dysplasia and carcinomas complicating ulcerative colitis. British Journal of Cancer, 64 (1). pp. 174-178. ISSN 0007-0920
Abstract
One hundred and nine samples comprising carcinomas, adenomas, dysplastic, inflamed and normal mucosa from patients with sporadic colon cancer and ulcerative colitis (UC) were analysed for c-Ki-ras mutations. DNA was extracted from archival paraffin-embedded material, amplified using the polymerase chain reaction (PCR) and the PCR products analysed using restriction enzyme digestion. Forty-two per cent (14/33) of the sporadic carcinoma controls contained Ki-ras codon 12 mutations in contrast to 24% (8/33) of ulcerative colitis carcinomas. A significantly higher c-Ki-ras mutation rate was observed in rectal carcinomas (72%) in comparison to colonic carcinomas (28%) in control patients (P less than 0.04), while the opposite was observed in UC patients. The difference between the incidence of c-Ki-ras mutations in rectal carcinomas in UC (9%) and in sporadic rectal carcinomas (72%) was also significant (P less than 0.01). This lower prevalence rate and different site distribution of c-Ki-ras mutations in UC carcinomas compared to sporadic carcinomas suggests that specific genetic differences may underlie the causation of carcinomas arising in these situations.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Keywords: | Codon; Colitis, Ulcerative; Colon; Colonic Neoplasms; DNA, Neoplasm; Genes, ras; Humans; Mutation; Neoplasm Staging; Polymerase Chain Reaction; Rectal Neoplasms; Restriction Mapping |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > Institute of Molecular Medicine (LIMM) (Leeds) > Section of Opthalmology and Neurosciences (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cancer and Pathology (LICAP) > Pathology & Tumour Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 15 Jul 2019 09:58 |
Last Modified: | 15 Jul 2019 09:58 |
Status: | Published |
Publisher: | Springer Nature |
Identification Number: | 10.1038/bjc.1991.264 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:118843 |