Stevenson, SR, Kamisugi, Y orcid.org/0000-0002-5265-3658, Trinh, CH et al. (15 more authors) (2016) Genetic analysis of Physcomitrella patens identifies ABSCISIC ACID NON-RESPONSIVE, a regulator of ABA responses unique to basal land plants and required for desiccation tolerance. Plant Cell, 28 (6). pp. 1310-1327. ISSN 1040-4651
Abstract
The anatomically simple plants that first colonised land must have acquired molecular and biochemical adaptations to drought stress. Abscisic acid (ABA) coordinates responses leading to desiccation tolerance in all land plants. We identified ABA non-responsive mutants in the model bryophyte Physcomitrella patens and genotyped a segregating population to map and identify the ABA NON-RESPONSIVE (ANR) gene encoding a modular protein kinase comprising an N-terminal PAS domain, a central EDR domain and a C-terminal MAPKKK like domain. Ppanr mutants fail to accumulate dehydration tolerance-associated gene products in response to drought, ABA or osmotic stress, and do not acquire ABA-dependent desiccation tolerance. The crystal structure of the PAS domain, determined to 1.7Å resolution, shows a conserved PAS-fold, and to dimerise with a weak dimerization interface. Targeted mutagenesis of a conserved tryptophan residue within the PAS domain generates plants with ABA non-responsive growth and strongly attenuated ABA-responsive gene expression, whereas deleting this domain retains a fully ABA responsive phenotype. ANR orthologs are found in early-diverging land plant lineages and aquatic algae, but are absent from more recently diverged vascular plants. We propose that ANR genes represent an ancestral adaptation enabling drought-stress survival of the first terrestrial colonisers, but were lost during land plant evolution.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2016 The author(s). This is an open access article under the terms of the Creative Commons CC-BY 4.0 license, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Abscisic Acid; Physcomitrella patens; Desiccation tolerance; MAP3 Kinase; Targeted mutagenesis; Genetic mapping; Land plant evolution |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Crystallography (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) > Synthetic Biology (Leed) |
Funding Information: | Funder Grant number BBSRC BB/F001797/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 10 May 2016 09:30 |
Last Modified: | 17 Jan 2020 15:20 |
Published Version: | https://dx.doi.org/10.1105/tpc.16.00091 |
Status: | Published |
Publisher: | American Society of Plant Biologists |
Identification Number: | 10.1105/tpc.16.00091 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:99402 |