Noctor, G, Mhamdi, A and Foyer, CH (2016) Oxidative stress and antioxidative systems: recipes for successful data collection and interpretation. Plant, Cell and Environment, 39 (5). pp. 1140-1160. ISSN 0140-7791
Abstract
Oxidative stress and reactive oxygen species (ROS) are common to many fundamental responses of plants. Enormous and ever-growing interest has focused on this research area, leading to an extensive literature that documents the tremendous progress made in recent years. As in other areas of plant biology, advances have been greatly facilitated by developments in genomics-dependent technologies and the application of interdisciplinary techniques that generate information at multiple levels. At the same time, advances in understanding ROS are fundamentally reliant on the use of biochemical and cell biology techniques that are specific to the study of oxidative stress. It is therefore timely to revisit these approaches with the aim of providing a guide to convenient methods and assisting interested researchers in avoiding potential pitfalls. Our critical overview of currently popular methodologies includes a detailed discussion of approaches used to generate oxidative stress, measurements of ROS themselves, determination of major antioxidant metabolites, assays of antioxidative enzymes, and marker transcripts for oxidative stress. We consider the applicability of metabolomics, proteomics, and transcriptomics approaches, and discuss markers such as damage to DNA and RNA. Our discussion of current methodologies is firmly anchored to future technological developments within this popular research field.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2016 John Wiley & Sons Ltd. This is the peer reviewed version of the following article: Noctor, G., Mhamdi, A., and Foyer, C. H. (2016) Oxidative stress and antioxidative systems: recipes for successful data collection and interpretation. Plant, Cell & Environment, 39: 1140–1160. doi: 10.1111/pce.12726, which has been published in final form at http://dx.doi.org/10.1111/pce.12726. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
Keywords: | Reactive oxygen species (ROS); hydrogen peroxide; glutathione; ascorbate; NAD(P); antioxidative enzymes; redox proteomics; lipid peroxides; DNA damage |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biology (Leeds) |
Funding Information: | Funder Grant number BBSRC BB/M009130/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Feb 2016 12:45 |
Last Modified: | 01 Jul 2017 21:20 |
Published Version: | http://dx.doi.org/10.1111/pce.12726 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1111/pce.12726 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:94570 |