Kirkwood, Jobie, Wilson, Julie orcid.org/0000-0002-5171-8480, O'keefe, Simon orcid.org/0000-0001-5957-2474 et al. (1 more author) (2014) A high-throughput colourimetric method for the determination of pH in crystallization screens. Acta Crystallographica. Section D, Biological Crystallography. pp. 2367-2375. ISSN 1399-0047
Abstract
The crystallization of proteins is dependent on the careful control of numerous parameters, one of these being pH. The pH of crystallization is generally reported as that of the buffer; however, the true pH has been found to be as many as four pH units away. Measurement of pH with a meter is time-consuming and requires the reformatting of the crystallization solution. To overcome this, a high-throughput method for pH determination of buffered solutions has been developed with results comparable to those of a pH meter.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2014 International Union of Crystallography. Reproduced in accordance with the publisher's self-archiving policy. |
Keywords: | absorbance,bromothymol blue,colourimetry,crystallization,indicator dye,pH,spectrophotometry |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Computer Science (York) The University of York The University of York > Faculty of Sciences (York) > Chemistry (York) The University of York > Faculty of Sciences (York) > Mathematics (York) |
Depositing User: | Pure (York) |
Date Deposited: | 10 Dec 2015 13:57 |
Last Modified: | 26 Jan 2025 00:08 |
Published Version: | https://doi.org/10.1107/S1399004714014011 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1107/S1399004714014011 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:92630 |