Kroeger, JK, Hassan, K, Voros, A et al. (7 more authors) (2015) Bacillus cereus efflux protein BC3310 – a multidrug transporter of the unknown major facilitator family, UMF-2. Frontiers in Microbiology, 6. ISSN 1664-302X
Abstract
Phylogenetic classification divides the major facilitator superfamily (MFS) into 82 families, including 25 families that are comprised of transporters with no characterized functions. This study describes functional data for BC3310 from Bacillus cereus ATCC 14579, a member of the “unknown major facilitator family-2” (UMF-2). BC3310 was shown to be a multidrug efflux pump conferring resistance to ethidium bromide, SDS and silver nitrate when heterologously expressed in Escherichia coli DH5α acrAB. A conserved aspartate residue (D105) in putative transmembrane helix 4 was identified, which was essential for the energy dependent ethidium bromide efflux by BC3310. Transport proteins of the MFS comprise specific sequence motifs. Sequence analysis of UMF- 2 proteins revealed that they carry a variant of the MFS motif A, which may be used as a marker to distinguish easily between this family and other MFS proteins. Genes orthologous to bc3310 are highly conserved within the B. cereus group of organisms and thus belong to the core genome, suggesting an important conserved functional role in the normal physiology of these bacteria.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 Kroeger, Hassan, Vörös, Simm, Saidijam, Bettaney, Bechthold, Paulsen, Henderson and Kolstø. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
Keywords: | MFS, drug resistance, efflux protein, Bacillus cereus, UMF-2 |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Funding Information: | Funder Grant number EU - European Union 201924 EU - European Union PIRSES-GA-2009-247634 |
Depositing User: | Symplectic Publications |
Date Deposited: | 15 Oct 2015 10:57 |
Last Modified: | 15 Oct 2015 10:57 |
Published Version: | http://dx.doi.org/10.3389/fmicb.2015.01063 |
Status: | Published |
Publisher: | Frontiers |
Identification Number: | 10.3389/fmicb.2015.01063 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:90776 |