Arafat, MT, Tronci, G orcid.org/0000-0002-9426-4220, Yin, J et al. (2 more authors) (2015) Biomimetic wet-stable fibres via wet spinning and diacid-based crosslinking of collagen triple helices. Polymer, 77. pp. 102-112. ISSN 0032-3861
Abstract
One of the limitations of electrospun collagen as bone-like fibrous structure is the potential
collagen triple helix denaturation in the fibre state and the corresponding inadequate wet
stability even after crosslinking. Here, we have demonstrated the feasibility of accomplishing
wet-stable fibres by wet spinning and diacid-based crosslinking of collagen triple helices,
whereby fibre ability to act as bone-mimicking mineralisation system has also been explored.
Circular dichroism (CD) demonstrated nearly complete triple helix retention in resulting wetspun
fibres, and the corresponding chemically crosslinked fibres successfully preserved their
fibrous morphology following 1-week incubation in phosphate buffer solution (PBS). The
presented novel diacid-based crosslinking route imparted superior tensile modulus and
strength to the resulting fibres indicating that covalent functionalization of distant collagen
molecules is unlikely to be accomplished by current state-of-the-art carbodiimide-based
crosslinking. To mimic the constituents of natural bone extra cellular matrix (ECM), the
crosslinked fibres were coated with carbonated hydroxyapatite (CHA) through biomimetic
precipitation, resulting in an attractive biomaterial for guided bone regeneration (GBR), e.g.
in bony defects of the maxillofacial region.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | collagen; wet spinning; fibres; biopolymer; collagen crosslinking; carbonated hydroxyapatite; bone |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) The University of Leeds > Faculty of Performance, Visual Arts and Communications (Leeds) |
Funding Information: | Funder Grant number EPSRC (Engineering and Physical Sciences Research Council) EP/K029592/1 The Clothworkers Foundation ANDREW BLESSLEY EPSRC (Engineering and Physical Sciences Research Council) Not Known EPSRC (Engineering and Physical Sciences Research Council) Not Known |
Depositing User: | Symplectic Publications |
Date Deposited: | 22 Sep 2015 10:38 |
Last Modified: | 02 Dec 2020 21:23 |
Published Version: | http://dx.doi.org/10.1016/j.polymer.2015.09.037 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.polymer.2015.09.037 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:90093 |