Fermor, HL, McLure, SWD, Taylor, SD et al. (4 more authors) (2015) Biological, biochemical and biomechanical characterisation of articular cartilage from the porcine, bovine and ovine hip and knee. Bio-Medical Materials and Engineering, 25 (4). 381 - 395. ISSN 0959-2989
Abstract
This study aimed to determine the optimal starting material for the development of an acellular osteochondral graft. Osteochondral tissues from three different species were characterised; pig (6 months), cow (18 months) and two ages of sheep (8 - 12 months and > 4 year old). Tissues from the acetabulum and femoral head of the hip, and the groove, medial and lateral condyles and tibial plateau of the knee were assessed. Histological analysis of each tissue allowed for qualification of cartilage histoarchitecture, glycosaminoglycan (GAG) distribution, assessment of cellularity and cartilage thickness. Collagen and GAG content were quantified and cartilage water content was defined. Following biomechanical testing, the percentage deformation, permeability and equilibrium elastic modulus was determined. Results showed that porcine cartilage had the highest concentration of sulphated proteoglycans and that the condyles and groove of the knee showed higher GAG content than other joint areas. Cartilage from younger tissues (porcine and young ovine) had higher cell content and was thicker, reflecting the effects of age on cartilage structure. Cartilage from older sheep had a much higher elastic modulus and was less permeable than other species.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015, IOS Press. This is an author produced version of a paper published in Bio-Medical Materials and Engineering. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Cartilage; Glycosaminoglycan; Collagen; Thickness; Water Content; Cellularity; Percentage Deformation; Permeability; Equilibrium Elastic Modulus |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Mechanical Engineering (Leeds) > Institute of Medical and Biological Engineering (iMBE) (Leeds) |
Funding Information: | Funder Grant number Wellcome Trust 088908/Z/09/Z |
Depositing User: | Symplectic Publications |
Date Deposited: | 06 Nov 2015 13:40 |
Last Modified: | 17 Jan 2018 16:09 |
Published Version: | http://dx.doi.org/10.3233/BME-151533 |
Status: | Published |
Publisher: | IOS Press |
Identification Number: | 10.3233/BME-151533 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:88649 |