Tronci, G, Kanuparti, R, Arafat, MT et al. (3 more authors) (2015) Wet-spinnability and crosslinked fibre properties of two collagen polypeptides with varied molecular weight. International Journal of Biological Macromolecules, 81. pp. 112-120. ISSN 0141-8130
Abstract
The formation of naturally-derived materials with wet stable fibrous architectures is paramount in order to mimic the features of tissues at the molecular and microscopic scale. Here, we investigated the formation of wet-spun fibres based on collagen-derived polypeptides with comparable chemical composition and varied molecular weight. Gelatin and hydrolysed fish collagen (HFC) were selected as widely-available linear amino-acidic chains of high and low molecular weight, respectively, and functionalised in the wet-spun fibre state in order to preserve the material geometry in physiological conditions. Wet-spun fibre diameter and morphology were dramatically affected depending on the polypeptide molecular weight, wet-spinning solvent (i.e. 2,2,2-Trifluoroethanol and dimethyl sulfoxide) and coagulating medium (i.e. acetone and ethanol), resulting in either bulky or porous internal geometry. Dry-state tensile moduli were significantly enhanced in gelatin and HFC samples following covalent crosslinking with activated 1,3-phenylenediacetic acid (Ph) (E: 726 ± 43 ‒ 844 ± 85 MPa), compared to samples crosslinked via intramolecular carbodiimide-mediated condensation reaction (E: 588 ± 38 MPa). Resulting fibres displayed a dry diameter in the range of 238±18–355±28 μm and proved to be mechanically-stable (E: 230 kPa) following equilibration with PBS, whilst a nearly-complete degradation was observed after 5-day incubation in physiological conditions.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015, Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Polypeptide; collagen; gelatin; wet-spinning; fibre; crosslink density |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) |
Funding Information: | Funder Grant number Clothworkers ANDREW BLESSLEY |
Depositing User: | Symplectic Publications |
Date Deposited: | 30 Jul 2015 10:41 |
Last Modified: | 26 Feb 2019 09:41 |
Published Version: | http://dx.doi.org/10.1016/j.ijbiomac.2015.07.053 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.ijbiomac.2015.07.053 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:88536 |