Meitha, K, Konnerup, D, Colmer, TD et al. (3 more authors) (2015) Spatio-temporal relief from hypoxia and production of reactive oxygen species during bud burst in grapevine (Vitis vinifera L.). Annals of Botany, 116 (4). 703 - 711. ISSN 0305-7364
Abstract
Background and Aims: Plants regulate cellular oxygen partial pressures (pO2), together with reduction/ oxidation (redox) state to manage rapid developmental transitions such as bud burst after a period of quiescence. However, our understanding of pO2 regulation in complex meristematic organs such as buds is incomplete, and particularly lacks spatial resolution. Methods: The gradients in pO2 from the outer scales to the primary meristem complex were measured in grapevine (Vitis vinifera L.) buds, together with respiratory CO2 production rates and the accumulation of superoxide and hydrogen peroxide, from ecodormancy through the first 72 h preceding bud burst, triggered by the transition from low to ambient temperatures. Key Results: Steep internal pO2 gradients were measured in dormant buds with values as low as 2.5 kPa found in the core of the bud prior to bud burst. Respiratory CO2 production rates increased soon after the transition from low to ambient temperatures and the bud tissues gradually became oxygenated in a patterned process. Within 3 h of the transition to ambient temperatures, superoxide accumulation was observed in the cambial meristem, co-localising with lignified cellulose associated with pro-vascular tissues. Thereafter, superoxide accumulated in other areas subtending the apical meristem complex, in the absence of significant hydrogen peroxide accumulation, except in the cambial meristem. By 72 h, the internal pO2 gradient showed a biphasic profile, where the minimum pO2 was external to the core of the bud complex. Conclusions: Spatial and temporal control of the tissue oxygen environment occurs within quiescent buds, and the transition from quiescence to bud burst is accompanied by a regulated relaxation of the hypoxic state and accumulation of reactive oxygen species (ROS) within the developing cambium and vascular tissues of the heterotrophic grapevine buds.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Author(s), 2015. Published by Oxford University Press on behalf of the Annals of Botany Company. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Bud burst; Vitis vinifera L.; Grapevine; Reactive Oxygen Species; Superoxide; Hypoxia; Oxygen Partial Pressure; Meristem; Development; Respiration; Ecodormancy; Quiescence. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) |
Funding Information: | Funder Grant number Royal Society IE111477 Australian Research Council Not Known |
Depositing User: | Symplectic Publications |
Date Deposited: | 15 Jul 2015 12:19 |
Last Modified: | 17 Jan 2018 07:39 |
Published Version: | http://dx.doi.org/10.1093/aob/mcv123 |
Status: | Published |
Publisher: | Oxford University Press |
Identification Number: | 10.1093/aob/mcv123 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:87962 |