Landels, A., Evans, C., Noirel, J. et al. (1 more author) (2015) Advances in proteomics for production strain analysis. Current Opinion in Biotechnology, 35. 111 - 117. ISSN 0958-1669
Abstract
Proteomics is the large-scale study and analysis of proteins, directed to analysing protein function in a cellular context. Since the vast majority of the processes occurring in a living cell rely on protein activity, proteomics offer a unique vantage point from which researchers can dissect, characterise, understand and manipulate biological systems. When developing a production strain, proteomics offers a versatile toolkit of analytical techniques. In this commentary, we highlight a number of recent developments in this field using three industrially relevant case studies: targeted proteomic analysis of heterologous pathways in Escherichia coli, biofuel production in Synechocystis PCC6803 and proteomic investigations of lignocellulose degradation. We conclude by discussing future developments in proteomics that will impact upon metabolic engineering and process monitoring of bio-producer strains.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2015 Elsevier. This is an author-produced version of a paper accepted for publication in Current Opinion in Biotechnology. Uploaded in accordance with the publisher's self-archiving policy. Available under the CC-BY-NC-ND licence (https://creativecommons.org/licenses/by-nc-nd/3.0/) |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Chemical and Biological Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 07 Jul 2015 08:02 |
Last Modified: | 04 Nov 2017 16:44 |
Published Version: | http://dx.doi.org/10.1016/j.copbio.2015.05.001 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.copbio.2015.05.001 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:87279 |