Fayed, Bahgat, Younger, Ellen, Taylor, Gabrielle et al. (1 more author) (2014) A novel Streptomyces spp. integration vector derived from the S. venezuelae phage, SV1. BMC Biotechnology. 51. ISSN 1472-6750
Abstract
Background: Integrating vectors based on the int/attP loci of temperate phages are convenient and used widely, particularly for cloning genes in Streptomyces spp. Results: We have constructed and tested a novel integrating vector based on g27, encoding integrase, and attP site from the phage, SV1. This plasmid, pBF3 integrates efficiently in S. coelicolor and S. lividans but surprisingly fails to generate stable integrants in S. venezuelae, the natural host for phage SV1. Conclusion: pBF3 promises to be a useful addition to the range of integrating vectors currently available for Streptomyces molecular genetics.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
Keywords: | Streptomyces,Cloning,Integration vector,Serine integrase,Bacteriophage,SV1 |
Dates: |
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Institution: | The University of York |
Academic Units: | The University of York > Faculty of Sciences (York) > Biology (York) |
Funding Information: | Funder Grant number BBSRC (BIOTECHNOLOGY AND BIOLOGICAL SCIENCES RESEARCH COUNCIL) BB/K003356/1 |
Depositing User: | Pure (York) |
Date Deposited: | 13 Nov 2014 17:50 |
Last Modified: | 07 Dec 2024 00:10 |
Published Version: | https://doi.org/10.1186/1472-6750-14-51 |
Status: | Published |
Refereed: | Yes |
Identification Number: | 10.1186/1472-6750-14-51 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:81670 |
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Description: A novel Streptomyces spp integration vector derived from the S. venezuelae phage, SV1