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Kljun, A, El-Dessouky, HM, Benians, TAS et al. (4 more authors) (2014) Analysis of the physical properties of developing cotton fibres. European Polymer Journal, 51. 57 - 68. ISSN 0014-3057
Abstract
Cotton fibres develop over four stages: initiation, elongation, secondary-wall thickening, and maturation. They develop a significant crystalline structure during the secondary wall thickening stage of development. Cotton fibres were harvested from 17 days to 60 days after flowering (dpa). Transmission Electron Microscopy (TEM), Interferometry, Attenuated Total Reflectance Fourier–transform Infrared (ATR–FTIR) spectroscopy, immunofluorescence labelling, and fluorescence spectroscopy were used to characterise the cotton fibres in different stages. It was found that, secondary wall thickening and micronaire remain fairly constant from 17 to 24 dpa, after that time significant change occurs until maturity. Maturity ratio increases as the fibres develop. Birefringence increases rapidly from 17 dpa to 26 dpa, then levels off up to 60 dpa. It is evident by comparing the lateral order index (LOI) and results from the binding of a crystalline-cellulose binding probe (CBM3a) that there is a significant increase in the degree of cellulose crystallinity from 17 dpa to 26 dpa. Hydrogen Bond Intensity (HBI) increased to 24 dpa and decreased from 24 to 40 dpa indicating significant changes in inter-molecular hydrogen bonds. From 40 to 60 dpa an increase of HBI was observed. It is concluded that during the maturation stage of cotton fibre development, water loss from lumen allows the cellulose chains to come closer together and to form intermolecular hydrogen-bonds. TEM, Interferometry, ATR–FTIR spectroscopy, and immunofluorescence labelling combined with fluorescence spectroscopy, were demonstrated to be useful techniques in quantifying physical changes in cotton fibres during development, offering advantages over traditional analytical techniques.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2014. Elsevier. Uploaded in accordance with the publisher's self-archiving policy. NOTICE: this is the author’s version of a work that was accepted for publication in European Polymer Journal. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European Polymer Journal , 51,(2014) DOI:10.1016/j.eurpolymj.2013.11.016 |
Keywords: | Cotton fibres; Maturity; Birefringence; ATR–FTIR; Crystallinity |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 18 Nov 2014 11:18 |
Last Modified: | 21 Apr 2015 19:57 |
Published Version: | http://dx.doi.org/10.1016/j.eurpolymj.2013.11.016 |
Status: | Published |
Publisher: | Elsevier |
Refereed: | Yes |
Identification Number: | 10.1016/j.eurpolymj.2013.11.016 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:81245 |
Available Versions of this Item
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Analysis of the physical properties of developing cotton fibres. (deposited 16 Sep 2014 11:40)
- Analysis of the physical properties of developing cotton fibres. (deposited 18 Nov 2014 11:18) [Currently Displayed]