Abou-Saleh, RH orcid.org/0000-0002-8471-2659, Swain, M, Evans, SD orcid.org/0000-0001-8342-5335 et al. (1 more author) (2014) Poly(ethylene glycol) lipid-shelled microbubbles: abundance, stability, and mechanical properties. Langmuir, 30 (19). pp. 5557-5563. ISSN 0743-7463
Abstract
Poly(ethylene glycol) (PEG) is widely used on the outside of biomedical delivery vehicles to impart stealth properties. Encapsulated gas microbubbles (MBs) are being increasingly considered as effective carriers for therapeutic intervention to deliver drug payloads or genetic vectors. MBs have the advantage that they can be imaged and manipulated by ultrasound fields with great potential for targeted therapy and diagnostic purposes. Lipid-shelled MBs are biocompatible and can be functionalized on the outer surface for tissue targeting and new therapeutic methods. As MBs become a key route for drug delivery, exploring the effect of PEG-ylation on the MB properties is important. Here, we systematically investigate the effect of PEG-lipid solution concentration ranging between 0 and 35 mol % on the formation of MBs in a microfluidic flow-focusing device. The abundance of the MBs is correlated with the MB lifetime and the whole MB mechanical response, as measured by AFM compression using a tipless cantilever. The maximal MB concentration and stability (lifetime) occurs at a low concentration of PEG-lipid (∼5 mol %). For higher PEG-lipid concentrations, the lifetime and MB concentration decrease, and are accompanied by a correlation between the predicted surface PEG configuration and the whole MB stiffness, as measured at higher compression loads. These results inform the rationale design and fabrication of lipid-based MBs for therapeutic applications and suggest that only relatively small amounts of PEG incorporation are required for optimizing MB abundance and stability while retaining similar mechanical response at low loads.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | (c) 2014, American Chemical Society. Uploaded in accordance with the publisher's self-archiving policy. This document is the unedited author's version of a Submitted Work that was subsequently accepted for publication in Langmuir, copyright © American Chemical Society after peer review. To access the final edited and published work, see http://pubs.acs.org/articlesonrequest/AOR-wRhKKTJetjdzTdHps2Pb |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Oct 2014 09:06 |
Last Modified: | 17 May 2019 08:53 |
Published Version: | http://dx.doi.org/10.1021/la404804u |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/la404804u |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:80883 |