Tronci, G, Ajiro, H, Russell, SJ orcid.org/0000-0003-0339-9611 et al. (2 more authors) (2014) Tuneable drug-loading capability of chitosan hydrogels with varied network architectures. Acta Biomaterialia, 10 (2). pp. 821-830. ISSN 1742-7061
Abstract
Advanced bioactive systems with defined macroscopic properties and spatio-temporal sequestration of extracellular biomacromolecules are highly desirable for next generation therapeutics. Here, chitosan (CT) hydrogels were prepared with neutral or negatively charged cross-linkers in order to promote selective electrostatic complexation with charged drugs. CT was functionalized with varied dicarboxylic acids, such as tartaric acid, poly(ethylene glycol) bis(carboxymethyl) ether, 1,4-phenylenediacetic acid and 5-sulfoisophthalic acid monosodium salt (PhS), whereby PhS was hypothesized to act as a simple mimetic of heparin. Attenuated total reflectance Fourier transform infrared spectroscopy showed the presence of Cdouble bond; length as m-dashO amide I, N–H amide II and Cdouble bond; length as m-dashO ester bands, providing evidence of covalent network formation. The cross-linker content was reversely quantified by proton nuclear magnetic resonance on partially degraded network oligomers, so that 18 mol.% PhS was exemplarily determined. Swellability (SR: 299 ± 65–1054 ± 121 wt.%), compressibility (E: 2.1 ± 0.9–9.2 ± 2.3 kPa), material morphology and drug-loading capability were successfully adjusted based on the selected network architecture. Here, hydrogel incubation with model drugs of varied electrostatic charge, i.e. allura red (AR, doubly negatively charged), methyl orange (MO, negatively charged) or methylene blue (MB, positively charged), resulted in direct hydrogel–dye electrostatic complexation. Importantly, the cationic compound, MB, showed different incorporation behaviours, depending on the electrostatic character of the selected cross-linker. In light of this tunable drug-loading capability, these CT hydrogels would be highly attractive as drug reservoirs towards e.g. the fabrication of tissue models in vitro.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2013 Acta Materialia Inc. Published by Elsevier Ltd. Uploaded in accordance with the publisher's self-archiving policy. NOTICE: this is the author’s version of a work that was accepted for publication in Acta Biomaterialia. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Acta Biomaterialia, 10, 2, (2014) DOI 10.1016/j.actbio.2013.10.014 |
Keywords: | Chitosan; bioactive hydrogels; drug loading; sulfonic acid; crosslinked network |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Oct 2014 16:26 |
Last Modified: | 03 Nov 2016 02:40 |
Published Version: | http://dx.doi.org/10.1016/j.actbio.2013.10.014 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.actbio.2013.10.014 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:80783 |