Hatton, C.J. and Peers, C. (1997) Electrochemical detection of K+-evoked quantal secretory events from isolated rat type I carotid body cells. Experimental Physiology, 82 (2). pp. 415-418. ISSN 0958-0670
Abstract
Using amperometric techniques, electrochemical events associated with vesicular transmitter release were recorded from isolated rat type I carotid body cells when exposed to a solution containing 50mm K+. Events were enhanced in amplitude by preloading cells with the catecholamine precursor, L-b-3,4-dihydroxyphenylalanine (L-DOPA). K+-evoked secretion was abolished by the non-selective Ca2, channel blocker Cd2+ (100 ,/M) and markedly reduced by the L-type Ca2+ channel blocker nifedipine (5 µM). Our results indicate that secretion from isolated rat type I cells can be monitored electrochemically and we demonstrate a major role for L-type Ca2+ channels in mediating K+-evoked secretion.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 1997 The Physiological Society. Paper freely available from the Experimental Physiology website. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Genetics, Health and Therapeutics (LIGHT) > Academic Unit of Cardiovascular Medicine (Leeds) |
Depositing User: | Repository Officer |
Date Deposited: | 28 Sep 2007 09:16 |
Last Modified: | 18 Jun 2015 17:25 |
Published Version: | http://ep.physoc.org/cgi/reprint/82/2/415.pdf |
Status: | Published |
Publisher: | Blackwell Publishing |
Refereed: | Yes |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:3381 |