Role of intra-lamellar collagen and hyaluronan nanostructures in annulus fibrosus on lumbar spine biomechanics: insights from molecular mechanics-finite element–based multiscale analyses

Annulus fibrosus’ (AF) ability to transmit multi-directional spinal motion is contributed by a combination of chemical interactions among biomolecular constituents—collagen type I (COL-I), collagen type II (COL-II), and proteoglycans (aggrecan and hyaluronan)—and mechanical interactions at multiple length scales. However, the mechanistic role of such interactions on spinal motion is unclear. The present work employs a molecular mechanics-finite element (FE) multiscale approach to investigate the mechanistic role of molecular-scale collagen and hyaluronan nanostructures in AF, on spinal motion. For this, an FE model of the lumbar segment is developed wherein a multiscale model of AF collagen fiber, developed from COL-I, COL-II, and hyaluronan using the molecular dynamics-cohesive finite element multiscale method, is incorporated. Analyses show AF collagen fibers primarily contribute to axial rotation (AR) motion, owing to angle-ply orientation. Maximum fiber strain values of 2.45% in AR, observed at the outer annulus, are 25% lower than the reported values. This indicates native collagen fibers are softer, attributed to the softer non-fibrillar matrix and higher interfibrillar sliding. Additionally, elastic zone stiffness of 8.61 Nm/° is observed to be 20% higher than the reported range, suggesting native AF lamellae exhibit lower stiffness, resulting from inter-collagen fiber bundle sliding. The presented study has further implications towards the hierarchy-driven designing of AF-substitute materials.