Atma, Y., Sadeghpour, A. orcid.org/0000-0002-0475-7858, Murray, B.S. orcid.org/0000-0002-6493-1547 et al. (1 more author) (2025) Chitosan-alginate polyelectrolyte complexes for encapsulation of low molecular weight fish bioactive peptides. Food Hydrocolloids, 160 (Part 1). 110789. ISSN 0268-005X
Abstract
Encapsulation of low molecular weight (Mw) bioactive peptides (BAPs) in electrostatically mediated polyelectrolyte complexes (PECs) of sodium alginate (ALG) and chitosan (CS) was studied, formed via a simple one-step mixing process. PECs were characterized via dynamic light scattering (DLS), mixed-mode phase analysis light scattering (M3-PALS), static light scattering (SLS), small-angle X-ray scattering (SAXS) and transmission electron microscopy (TEM). The encapsulation efficiency (EE) and in vitro release of low Mw antihypertensive LKPNM and LKP BAPs (derived from fish hydrolysates) were measured, under both gastric and intestinal pH conditions, via high performance liquid chromatography (HPLC). Two different ALG were tested (Mw ≈ 8 and 21 kDa, with mannuronic: guluronic ratios 5.1 and 1.4, respectively), whilst the Mw and degree of acetylation (DA) of the CS were ≈111 kDa and ≈10 %, respectively. At the pH (5.5) of PEC formation the BAPs were positively charged. As the molar charge ratio of alginate to chitosan (n−/n+) was increased from 0.1 to 0.6 the PEC size reduced from ca. 800 to 300 ± 50 nm, indicating more compact structures, but increased again significantly to >10 μm around charge neutralisation (n−/n+ = 1) and net PEC ζ-potential swapping from +ve to –ve. The size then decreased again to between 1 and 10 μm as n−/n+ → 10, as expected if the more prevalent polysaccharide (ALG) coated the surface of the PECs. However, higher Mw ALG gave significantly smaller (more compact) and more highly negatively charged PECs for n−/n+ > 1. The PECs showed high (≈80%) EE but low (10–20%) release in gastrointestinal conditions, highlighting their potential as effective carriers of such BAPs.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
Keywords: | Alginate; Chitosan; Simulated gastrointestinal conditions; Fish peptide; Polyelectrolyte complexes |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Colloids and Food Processing (Leeds) The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 29 Nov 2024 16:27 |
Last Modified: | 29 Nov 2024 16:27 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.foodhyd.2024.110789 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:220236 |