Warren, J. orcid.org/0000-0002-0039-5730, Coe, R., Culbert, M. et al. (5 more authors) (2024) Injectable peptide-glycosaminoglycan hydrogels for soft tissue repair: in vitro assessment for nucleus augmentation. Materials Advances, 5 (21). pp. 8665-8672. ISSN 2633-5409
Abstract
We report the development of peptide-glycosaminoglycan hydrogels as injectable biomaterials for load-bearing soft tissue repair. The hydrogels are injectable as a liquid for clinical delivery, rapidly form a gel in situ, and mimic the osmotic swelling behaviour of natural tissue. We used a new in vitro model to demonstrate their application as a nucleus augmentation material for the treatment of intervertebral disc degeneration. Our study compared a complex lab gel preparation method to a simple clinical benchtop process. We showed pH differences did not significantly affect gel formation, and temperature variations had no impact on gel performance. Rheological results demonstrated consistency after benchtop mixing or needle injection. In our in vitro disc degeneration model, we established that peptide augmentation could restore the native biomechanical properties. This suggests the feasibility of minimally invasive peptide-GAG gel delivery, maintaining consistent properties across temperature and needle sizes while restoring disc height and stiffness in vitro.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2024 The Author(s). This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 3.0). |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Mechanical Engineering (Leeds) > Institute of Medical and Biological Engineering (iMBE) (Leeds) |
Funding Information: | Funder Grant number EPSRC (Engineering and Physical Sciences Research Council) EP/K020757/1 EPSRC (Engineering and Physical Sciences Research Council) EP/N00941X/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 07 Oct 2024 14:30 |
Last Modified: | 29 Oct 2024 15:31 |
Status: | Published |
Publisher: | The Royal Society of Chemistry |
Identification Number: | 10.1039/D4MA00613E |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:217968 |