Guo, Z., Fu, X., Zhou, R. et al. (5 more authors) (2024) A silicon-based functional self-assembled aptasensor for the detection of aflatoxin B1 by SERS sensing. Analytical Methods. ISSN 1759-9660
Abstract
One of the most harmful contaminants found in corn and its products is aflatoxin B1 (AFB1) and thus developing reliable detection methods is of great significance to consumers and the food industry. In this research, AuMBA@Ag nanoparticles (NPs) and AgNPs deposited on a silicon wafer (Si@AgNPs) were functionalized with an aptamer and its complementary strand, respectively, and self-assembled into a SERS aptasensor, which generated strong SERS signals. AFB1 bound to the aptamer prior to the complementary chain, causing AuMBA@Ag NPs to detach from Si@AgNPs. The complex dissociated, leading to a decrease in signal intensity from the solid-phase substrate. Under optimal conditions, the linear detection range was 0.05–20.0 ng mL⁻¹, and the detection limit was 0.039 ng mL⁻¹. Notably, the aptasensor demonstrated a recovery rate between 92.77% and 110.13% when utilized for the detection of AFB1 in corn flour and oil, indicating its good potential for detecting AFB1 in real sample matrices. In conclusion, a quantitative and reliable specific SERS detection system for AFB1 was developed in this study with significant applicability to food safety.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © The Royal Society of Chemistry 2024. This is an author produced version of an article published in Analytical Methods. Uploaded in accordance with the publisher's self-archiving policy. |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Colloids and Food Processing (Leeds) The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Nutrition and Public Health (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 27 Sep 2024 09:23 |
Last Modified: | 27 Sep 2024 09:23 |
Status: | Published online |
Publisher: | Royal Society of Chemistry (RSC) |
Identification Number: | 10.1039/d4ay01266f |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:217676 |
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Filename: Anal. Methods, 2024.pdf
