Sahin, S.S. orcid.org/0000-0003-0961-8563, Hernández-Álvarez, A.J., Ke, L. orcid.org/0000-0002-4358-2210 et al. (3 more authors) (2024) Composition, characterisation and emulsifying properties of natural nanoparticles in chickpea aquafaba for the formation of chilli oleoresin-in-water Pickering emulsions. Food Hydrocolloids, 150. 109728. ISSN 0268-005X
Abstract
This study aims to assess the compositional characteristics of British Kabuli chickpea aquafaba (AF) by conducting a comprehensive analysis from raw chickpeas to centrifugation fractions of AF and its potential use in forming and stabilising capsaicin-loaded O/W Pickering systems by exploiting the presence of self-assembled nanoparticles and proteins, which act as natural-coating agent. To this end, chickpeas were soaked (16 h, 4 °C, 1:4 chickpea: water) and pressure-cooked (20 min, 113 °C, 10 psig, 1:5 chickpea: water). The dry weight-based (DWB) compositions included total carbohydrate (76.33 ± 4.20%), protein (16.29 ± 0.43%), total phenolics (7.05–8.77 mg/g) and saponins (39.95 ± 0.89 mg/g), thus confirming the leaching of these components from seeds to AF. SDS-PAGE electrophoresis analysis revealed the presence of low MW proteins (≤∼16 kDa). The monosaccharides comprised d-glucose, d-galactose, l-arabinose, d-xylose and d-fructose. AF's particle size distribution revealed the occurrence of a bimodal population of nanoparticles (Dh ≤∼1000 nm and Dh ≤∼100 nm), further characterised by SAXS and TEM imaging. O/W emulsions were prepared with three chilli oleoresin types (capsicum, chilli birds' eye, and chilli ancho) by high-pressure homogenisation. The emulsion with the highest capsaicin content (capsicum oleoresin) was the most stable while the emulsion with the lowest capsaicin content (chilli ancho oleoresin) was the least stable. The presence of incidental nanoparticles and denatured proteins in AF was reasoned to account for the formation and stabilisation of chilli oleoresin-in-water Pickering emulsions, a newly offered explanation for its interfacial properties that will be pursued further in future studies.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2024 The Authors. This is an open access article under the terms of the Creative Commons Attribution License (CC-BY 4.0), which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | Aquafaba; Chickpea; Encapsulation; Nanoparticle; Chilli oleoresin; Capsaicin |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Colloids and Food Processing (Leeds) The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Chemistry and Biochemistry (Leeds) The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 22 Mar 2024 09:37 |
Last Modified: | 22 Mar 2024 09:37 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.foodhyd.2024.109728 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:210718 |