This is the latest version of this eprint.
Heath, G.R. orcid.org/0000-0001-6431-2191, Micklethwaite, E. and Storer, T.M. (2024) NanoLocz: Image Analysis Platform for AFM, High-Speed AFM and Localization AFM. Small Methods. ISSN 2366-9608
Abstract
Atomic Force Microscopy (AFM), High-Speed AFM (HS-AFM) simulation AFM, and Localization AFM (LAFM) enable the study of molecules and surfaces with increasingly higher spatiotemporal resolution. However, effective and rapid analysis of the images and movies produced by these techniques can be challenging, often requiring the use of multiple image processing software applications and scripts. Here, NanoLocz, an open-source solution that offers advanced analysis capabilities for the AFM community, is presented. Integration and continued development of AFM analysis tools is essential to improve access to data, increase throughput, and open new analysis opportunities. NanoLocz efficiently leverages the rich data AFM has to offer by incorporating and combining existing and newly developed analysis methods for AFM, HS-AFM, simulation AFM, and LAFM seamlessly. It facilitates and streamlines AFM analysis workflows from import of raw data, through to various analysis workflows. Here, the study demonstrates the capabilities of NanoLocz and the new methods it enables including single-molecule LAFM, time-resolved LAFM, and simulation LAFM.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2024 The Authors. Small Methods published by Wiley-VCH GmbH.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
Keywords: | AFM; DNA origami; high-speed AFM; image processing; simulation AFM ; structural biology |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Physics and Astronomy (Leeds) > Molecular & Nanoscale Physics |
Funding Information: | Funder Grant number EPSRC (Engineering and Physical Sciences Research Council) EP/W034735/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Mar 2024 17:00 |
Last Modified: | 12 Mar 2024 10:38 |
Status: | Published online |
Publisher: | Wiley |
Identification Number: | 10.1002/smtd.202301766 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:209824 |
Available Versions of this Item
-
NanoLocz: Image analysis platform for AFM, high-speed AFM and localization AFM. (deposited 12 Mar 2024 10:34)
- NanoLocz: Image Analysis Platform for AFM, High-Speed AFM and Localization AFM. (deposited 04 Mar 2024 17:00) [Currently Displayed]