Paterson, S orcid.org/0000-0001-8974-5022, Holmes, WM and Rodgers, J (2022) Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse. PLOS Neglected Tropical Diseases, 16 (12). e0010962. ISSN 1935-2727
Abstract
Splenomegaly, an enlargement of the spleen, is a known clinical sign of the parasitic disease, human African trypanosomiasis. This study follows the development of splenomegaly in a group of mice over multiple infection points, using a non-invasive imaging modality, magnetic resonance imaging (MRI). CD-1 mice infected with GVR35 T.b. brucei demonstrated a significant increase in spleen size from day 7 post-infection, with changes in the spleen tracked in individual animals over five time points. At the final time point, the mean spleen weight calculated using the spleen volume from the MR images was compared with the post-mortem gross spleen weight. No significant difference was detected between the two methods (1.62 ± 0.06g using MRI and 1.51 ± 0.04g gross weight, p = 0.554). Haematology and histological analysis were also performed, giving additional insight into splenomegaly for the GVR35 strain of infection. The study demonstrates that MRI is a useful tool when examining changes in organ volume throughout HAT infection and may be applicable in the investigation of a range of conditions where changes in organ volume occur and MRI has not been used previously.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 Paterson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
Keywords: | Animals; Humans; Mice; Trypanosoma brucei brucei; Magnetic Resonance Imaging; Autopsy |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Mechanical Engineering (Leeds) > Institute of Medical and Biological Engineering (iMBE) (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 18 Jul 2023 10:54 |
Last Modified: | 18 Jul 2023 10:54 |
Status: | Published |
Publisher: | Public Library of Science |
Identification Number: | 10.1371/journal.pntd.0010962 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:201464 |