Li, K, Zou, H, Ettelaie, R orcid.org/0000-0002-6970-4650 et al. (2 more authors) (Cover date: April 3, 2023) Spatial Localization of Two Enzymes at Pickering Emulsion Droplet Interfaces for Cascade Reactions. Angewandte Chemie International Edition, 62 (15). e202300794. ISSN 1433-7851
Abstract
Developing biocatalytic cascades in abiological conditions is of utmost significance, but such processes often suffer from low reaction efficiency because of incompatible reaction environments and suppressed intermediate transportation. Herein we report a new type of biocatalytic cascade by localizing two different enzymes separately in the outer and inner interfacial layers of Pickering emulsion droplets. This versatile approach enables the localization of two enzymes in their preferred reaction microenvironments and simultaneously in nanoscale proximity of each other. The thus-designed interfacial biocatalytic cascades show outstanding catalytic efficiency in alkene epoxidation and thioether oxidation with in situ generation of hydrogen peroxide under mild conditions, 6.9–13.6 times higher than the catalytic efficiency of the free enzymes in solution and their multi-enzymatic counterparts. The remarkable interfacial effect of Pickering droplets was found to be responsible for the significantly enhanced cascading efficiency.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2023 Wiley-VCH GmbH. This is the peer reviewed version of the following article: Li, K., Zou, H., Ettelaie, R., Zhang, J., Yang, H., Angew. Chem. Int. Ed. 2023, 62, e202300794, which has been published in final form at https://doi.org/10.1002/anie.202300794. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
Keywords: | Biocatalysis; Cascade Reactions; Liquid–Liquid Interfaces; Oxidation; Pickering Emulsions |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Colloids and Food Processing (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 28 Mar 2023 10:14 |
Last Modified: | 15 Feb 2024 01:13 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/anie.202300794 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:197755 |