Wei, W, Ettelaie, R orcid.org/0000-0002-6970-4650, Zhang, X et al. (4 more authors) (2022) Co-compartmentalization of Enzymes and Cofactors within Pickering Emulsion Droplets for Continuous Flow Catalysis. Angewandte Chemie International Edition, 61 (45). e202211912. ISSN 1433-7851
Abstract
Co-immobilization of enzymes and cofactors in a manner suitable for use in continuous flow catalysis remains a great challenge because of the difficulty in ensuring the free accessibility of immobilized enzymes and cofactors. Herein, we present a continuous flow catalysis system based on co-compartmentalization of enzymes and cofactors within Pickering emulsion droplets, which can realize co-immobilized enzymes and cofactors and regeneration of cofactors within the droplets. As exemplified by enzyme-catalyzed ketone enantioselective reduction and enantioselective transamination, our systems exhibit long-term stability (300-400 h), outstanding total turnover number (TTN, 59204 mol mol -1 ) and several-fold enhancement in the enzyme catalytic efficiency (CE e ) in comparison to conventional biphasic reactions. Our method along with revealing insight into the co-compartmentalization effects will provide a vast opportunity for significantly advancing continuous flow biocatalysis towards the level of practical applications.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2022 Wiley-VCH GmbH. This is the peer reviewed version of the following article: Wei, W., Ettelaie, R., Zhang, X., Fan, M., Dong, Y., Li, Z. and Yang, H. (2022), Co-compartmentalization of Enzymes and Cofactors within Pickering Emulsion Droplets for Continuous Flow Catalysis. Angewandte Chemie International Edition, 61 (45). e202211912., which has been published in final form at https://doi.org/10.1002/anie.202211912. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited. |
Keywords: | Biphasic reactions; Continuous flow catalysis; Interfacial reactions; Pickering Emulsions |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Colloids and Food Processing (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 20 Sep 2022 14:39 |
Last Modified: | 16 Sep 2023 00:13 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/anie.202211912 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:191105 |