Catania, R, Machin, J, Rappolt, M orcid.org/0000-0001-9942-3035 et al. (3 more authors) (2022) Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs. Macromolecules, 55 (9). pp. 3415-3422. ISSN 0024-9297
Abstract
Hybrid vesicles (HVs) that consist of mixtures of block copolymers and lipids are robust biomimetics of liposomes, providing a valuable building block in bionanotechnology, catalysis, and synthetic biology. However, functionalization of HVs with membrane proteins remains laborious and expensive, creating a significant current challenge in the field. Here, using a new approach of extraction with styrene-maleic acid (SMA), we show that a membrane protein (cytochrome bo3) directly transfers into HVs with an efficiency of 73.9 ± 13.5% without the requirement of detergent, long incubation times, or mechanical disruption. Direct transfer of membrane proteins using this approach was not possible into liposomes, suggesting that HVs are more amenable than liposomes to membrane protein incorporation from a SMA lipid particle system. Finally, we show that this transfer method is not limited to cytochrome bo3 and can also be performed with complex membrane protein mixtures.
Metadata
Item Type: | Article |
---|---|
Authors/Creators: |
|
Copyright, Publisher and Additional Information: | © 2022 The Authors. Published by American Chemical Society. This is an open access article under the terms of the Creative Commons Attribution License (CC-BY). |
Dates: |
|
Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Physical Chemistry (Leeds) The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Colloids and Food Processing (Leeds) |
Funding Information: | Funder Grant number BBSRC (Biotechnology & Biological Sciences Research Council) BB/T000546/1 |
Depositing User: | Symplectic Publications |
Date Deposited: | 27 Apr 2022 13:53 |
Last Modified: | 25 Jun 2023 22:57 |
Status: | Published |
Publisher: | American Chemical Society |
Identification Number: | 10.1021/acs.macromol.2c00326 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:186081 |