Liang, H, Yin, J, Man, K et al. (6 more authors) (2022) A long-lasting guided bone regeneration membrane from sequentially functionalised photoactive atelocollagen. Acta Biomaterialia, 140. pp. 190-205. ISSN 1742-7061
Abstract
The fast degradation of collagen-based membranes in the biological environment remains a critical challenge, resulting in underperforming Guided Bone Regeneration (GBR) therapy leading to compromised clinical results. Photoactive atelocollagen (AC) systems functionalised with ethylenically unsaturated monomers, such as 4-vinylbenzyl chloride (4VBC), have been shown to generate mechanically competent materials for wound healing, inflammation control and drug delivery, whereby control of the molecular architecture of the AC network is key. Building on this platform, the sequential functionalisation with 4VBC and methacrylic anhydride (MA) was hypothesised to generate UV-cured AC hydrogels with reduced swelling ratio, increased proteolytic stability and barrier functionality for GBR therapy. The sequentially functionalised atelocollagen precursor (SAP) was characterised via TNBS and ninhydrin colourimetric assays, circular dichroism and UV-curing rheometry, which confirmed nearly complete consumption of collagen's primary amino groups, preserved triple helices and fast (< 180 s) gelation kinetics, respectively. Hydrogel's swelling ratio and compression modulus were adjusted depending on the aqueous environment used for UV-curing, whilst the sequential functionalisation of AC successfully generated hydrogels with superior proteolytic stability in vitro compared to both 4VBC-functionalised control and the commercial dental membrane Bio-Gide®. These in vitro results were confirmed in vivo via both subcutaneous implantation and a proof-of-concept study in a GBR calvarial model, indicating integrity of the hydrogel and barrier defect, as well as tissue formation following 1-month implantation in rats.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2021 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. This is an author produced version of an article published in Acta Biomaterialia. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | atelocollagen; barrier membrane; enzymatic stability; Guided Bone Regeneration; sequential functionalisation; UV curing |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) > Oral Biology (Leeds) |
Funding Information: | Funder Grant number EPSRC (Engineering and Physical Sciences Research Council) Not Known EPSRC (Engineering and Physical Sciences Research Council) Not Known EPSRC (Engineering and Physical Sciences Research Council) EP/R511717/1 The Clothworkers Company LEEDS39 |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Jan 2022 12:25 |
Last Modified: | 09 Dec 2022 01:13 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.actbio.2021.12.004 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:181291 |