Pabois, O orcid.org/0000-0001-5307-7149, Lorenz, CD, Harvey, RD et al. (5 more authors) (2019) Molecular insights into the behaviour of bile salts at interfaces: a key to their role in lipid digestion. Journal of Colloid and Interface Science, 556. pp. 266-277. ISSN 0021-9797
Abstract
Hypotheses
Understanding the mechanisms underlying lipolysis is crucial to address the ongoing obesity crisis and associated cardiometabolic disorders. Bile salts (BS), biosurfactants present in the small intestine, play key roles in lipid digestion and absorption. It is hypothesised that their contrasting functionalities – adsorption at oil/water interfaces and shuttling of lipolysis products away from these interfaces – are linked to their structural diversity. We investigate the interfacial films formed by two BS, sodium taurocholate (NaTC) and sodium taurodeoxycholate (NaTDC), differing by the presence or absence of a hydroxyl group on their steroid skeleton.
Experiments
Their adsorption behaviour at the air/water interface and interaction with a phospholipid monolayer – used to mimic a fat droplet interface – were assessed by surface pressure measurements and ellipsometry, while interfacial morphologies were characterised in the lateral and perpendicular directions by Brewster angle microscopy, X-ray and neutron reflectometry, and molecular dynamics simulations.
Findings
Our results provide a comprehensive molecular-level understanding of the mechanisms governing BS interfacial behaviour. NaTC shows a higher affinity for the air/water and lipid/water interfaces, and may therefore favour enzyme adsorption, whereas NaTDC exhibits a higher propensity for desorption from these interfaces, and may thus more effectively displace hydrolysis products from the interface, through dynamic exchange.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 Elsevier Inc. This is an author produced version of a paper published in Journal of Colloid and Interface Science. Uploaded in accordance with the publisher's self-archiving policy. This manuscript version is made available under the Creative Commons CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/. |
Keywords: | Bile salts; Lipid digestion; Interfacial properties; DPPC monolayer; Neutron reflectometry |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Food Science and Nutrition (Leeds) > FSN Colloids and Food Processing (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 26 Nov 2021 12:48 |
Last Modified: | 10 May 2022 01:21 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.jcis.2019.08.010 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:180881 |
Commentary/Response Threads
- Pabois, O, Lorenz, CD, Harvey, RD, Grillo, I, Grundy, MM-L, Wilde, PJ, Gerelli, Y and Dreiss, CA Molecular insights into the behaviour of bile salts at interfaces: a key to their role in lipid digestion. (deposited 26 Nov 2021 12:48) [Currently Displayed]
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