Qiu, L, Wu, J, Du, W et al. (5 more authors) (2021) Response of soil bacterial communities to sulfadiazine present in manure: Protection and adaptation mechanisms of extracellular polymeric substances. Journal of Hazardous Materials, 408. 124887. ISSN 0304-3894
Abstract
Extracellular polymeric substances (EPS) play a dominant role in protective biofilms. However, studies exploring the underlying protective mechanism of EPS have mainly focused on activated sludge, whereas their positive roles in protecting soil microbes from environmental stress have not been elucidated. In this study, we revealed the response of soil bacterial communities to various dosages of sulfadiazine (SDZ) present in manure, with a special emphasis on the role of EPS. Sequencing analysis showed that the bacterial community demonstrated stronger symbiotic relationships and weaker competitive interaction patterns to cope with disturbance induced by SDZ. EPS was mainly composed of tyrosine-like and tryptophan-like substances, and moreover, carboxyl, hydroxyl and ether groups were the main functional groups. An adaptation mechanism, namely the enhanced secretion of tryptophan-like substances, could help alleviate the SDZ stress effectively in the biofilms occurring in soil that experienced long-term manure application. Furthermore, the existence of EPS weakened the accumulation of antibiotic resistance genes (ARGs) in soil. Our results for the first time systematically uncover the joint action of biofilm tolerance and ARGs in resisting SDZ stress, which enhances understanding of the protective role of EPS and the underlying mechanisms governing biofilm functions in soil environments.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020, Elsevier B.V. All rights reserved. This is an author produced version of an article published in the Journal of Hazardous Materials. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | EPS; Antibiotic resistance; Sulfonamides |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Environment (Leeds) > School of Earth and Environment (Leeds) > Earth Surface Science Institute (ESSI) (Leeds) |
Funding Information: | Funder Grant number NERC (Natural Environment Research Council) NE/N007514/2 |
Depositing User: | Symplectic Publications |
Date Deposited: | 13 Jan 2021 16:04 |
Last Modified: | 17 Dec 2021 01:38 |
Status: | Published |
Publisher: | Elsevier BV |
Identification Number: | 10.1016/j.jhazmat.2020.124887 |
Related URLs: | |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:169563 |