Barba, FC, Chacón, MG, Reynolds, WR et al. (3 more authors) (2021) Improved conversion of residual MSW biomass waste to sugars using online process monitoring and integrated contamination control. Bioresource Technology Reports, 13. 100612. ISSN 2589-014X
Abstract
Enzymatic saccharification of lignocellulosic biomass to soluble sugars is vulnerable to microbial contamination. Identification of early stage indicators of contamination could allow for more rapid intervention and improved sugar retention. Here we validate the use of dissolved oxygen (DO) and pH as early-stage indicators, and used to evaluate the effectiveness of several antimicrobial agents (sodium azide, benzisothiazolinone, hydrogen peroxide and tetracycline). It was found that benzisothiazolinone (BIT) and sodium azide performed most favourably (8.93 and 9.89 g glucose/L, respectively), controlling contamination for the entire 24 hour time course assessed. We then describe the development of a novel integrated control system with online monitoring of DO/pH and automatic administration of a discrete doses of antimicrobial agent to the reaction once environmental DO dropped below a pre-defined threshold. Further, we show that FTIR can be used for continuous real-time glucose quantification during enzyme hydrolysis as an alternative to traditional offline measurement methods.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Published by Elsevier. This is an author produced version of a paper published in Bioresource Technology Reports. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Municipal solid waste (MSW); Enzymatic hydrolysis; Microbial growth indicators; Antimicrobial agents; Process monitoring |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemical & Process Engineering (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) |
Funding Information: | Funder Grant number Innovate UK fka Technology Strategy Board (TSB) 131844 Innovate UK fka Technology Strategy Board (TSB) Not Known |
Depositing User: | Symplectic Publications |
Date Deposited: | 04 Dec 2020 12:40 |
Last Modified: | 16 Jan 2023 09:52 |
Status: | Published |
Publisher: | Elsevier BV |
Identification Number: | 10.1016/j.biteb.2020.100612 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:168608 |