Gao, Z orcid.org/0000-0003-2808-0826, Hassouneh, L, Yang, X et al. (3 more authors) (2021) Hydrogen phosphate-mediated acellular biomineralisation within a dual crosslinked hyaluronic acid hydrogel. European Polymer Journal, 143. 110187. ISSN 0014-3057
Abstract
The creation of hyaluronic acid (HA)-based materials as biomineralisation scaffolds for cost-effective hard tissue regenerative therapies remains a key biomedical challenge. A non-toxic and simple acellular method to generate specific hydrogen phosphate (HPO42−) interactions within the polymer network of cystamine-crosslinked HA hydrogels is reported. Reinforced dual crosslinked hydrogel networks were accomplished after 4-week incubation in disodium phosphate-supplemented solutions that notably enabled the mineralisation of hydroxyapatite (HAp) crystals across the entire hydrogel structure. HPO42−–cystamine-crosslinked HA hydrogen bond interactions were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and density functional theory (DFT) calculations. HPO42−-mediated physical crosslinks proved to serve as a first nucleation step for acellular hydrogel mineralisation in simulated body fluid allowing HAp crystals to be detected by X-ray powder diffraction (2θ = 27°, 33° and 35°) and visualised with density gradient across the entire hydrogel network. On a cellular level, the presence of aggregated structures proved key to inducing ATDC 5 cell migration whilst no toxic response was observed after 3-week culture. This mild and facile ion-mediated stabilisation of HA-based hydrogels has significant potential for accelerated hard tissue repair in vivo and provides a new perspective in the design of dual crosslinked mechanically competent hydrogels.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 Elsevier Ltd. All rights reserved. This is an author produced version of an article published in European Polymer Journal. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | Hyaluronic acid; Hydrogel; Hydrogen phosphate interaction; Cystamine crosslinking; Biomineralisation |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Colour Science (Leeds) The University of Leeds > Faculty of Arts, Humanities and Cultures (Leeds) > School of Design (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Dentistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 30 Nov 2020 12:37 |
Last Modified: | 07 Jun 2022 00:38 |
Status: | Published |
Publisher: | Elsevier |
Identification Number: | 10.1016/j.eurpolymj.2020.110187 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:168540 |