Lizarraga Valderrama, L.D.R., Nigmatullin, R., Ladino, B. et al. (6 more authors) (2020) Modulation of neuronal cell affinity of composites scaffolds based on polyhydroxyalkanoates and bioactive glasses. Biomedical Materials, 15 (4). 045024. ISSN 1748-6041
Abstract
Biocompatibility and neuron regenerating properties of various bioactive glass (BG)/Polyhydroxyalkanoate (PHA) blend composites were assessed in order to study their suitability for peripheral nerve tissue applications, specifically as lumen structures for nerve guidance conduits (NGCs). BG/PHA blend composites were fabricated using Bioactive glass® 45S5 (BG1) and BG 1393 (BG2) with the 25:35 poly(3-hydroxyoctanoate/poly3-hydroxybutyrate), 25:75 P(3HO)/P(3HB) blend (PHA blend). Various concentrations of each BG (0.5, 1.0 and 2.5 wt%) were used to determine the effect of BG on neuronal growth and differentiation, in single culture using NG108-15 neuronal cells and in a co-culture along with RN22 Schwann cells. NG108-15 cells exhibited good growth and differentiation on all the PHA blend composites showing that both BGs have good biocompatibility at 0.5, 1.0 and 2.5 wt% within the PHA blend. The Young's modulus values displayed by all the PHA blend/BG composites ranged from 385.6 MPa to 1792.6 MPa, which are able to provide the required support and protective effect for regeneration of peripheral nerves. More specifically, the tensile strength obtained in the PHA blend/BG1 (1.0 wt%) (10.0 ± 0.6 MPa) was found to be similar to that of rabbit peroneal nerve. This composite also exhibited the best biological performance in supporting growth and neuronal differentiation among all the substrates. The neurite extension on this composite was found to be remarkable with the neurites forming a complex connection network.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2020 The Authors. As the Version of Record of this article is going to be/has been published on a gold open access basis under a CC BY 3.0 licence, this Accepted Manuscript is available for reuse under a CC BY 3.0 licence immediately. |
Keywords: | bioactive glasses; composite scaffolds; nerve regeneration; nerve tissue engineering; polyhydroxyalkanoates |
Dates: |
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Institution: | The University of Sheffield |
Academic Units: | The University of Sheffield > Faculty of Engineering (Sheffield) > Department of Materials Science and Engineering (Sheffield) |
Depositing User: | Symplectic Sheffield |
Date Deposited: | 19 May 2020 08:53 |
Last Modified: | 28 Oct 2021 11:19 |
Status: | Published |
Publisher: | IOP Publishing |
Refereed: | Yes |
Identification Number: | 10.1088/1748-605x/ab797b |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:160714 |
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