Fry, AM, Sampson, J orcid.org/0000-0002-0147-6014, Shak, C et al. (1 more author) (2017) Recent advances in pericentriolar material organization: ordered layers and scaffolding gels. F1000Research, 6. 1622. ISSN 2046-1402
Abstract
The centrosome is an unusual organelle that lacks a surrounding membrane, raising the question of what limits its size and shape. Moreover,while electron microscopy (EM) has provided a detailed view of centriole architecture, there has been limited understanding of how the second major component of centrosomes, the pericentriolar material (PCM), is organized. Here, we summarize exciting recent findings from super-resolution fluorescence imaging, structural biology, and biochemical reconstitution that together reveal the presence of ordered layers and complex gel-like scaffolds in the PCM. Moreover, we discuss how this is leading to a better understanding of the process of microtubule nucleation, how alterations in PCM size are regulated in cycling and differentiated cells, and why mutations in PCM components lead to specific human pathologies.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2017 Fry AM et al. This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
Keywords: | centrosomes, pericentriolar material (PCM), mitosis, |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Molecular and Cellular Biology (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 14 Feb 2020 16:05 |
Last Modified: | 14 Feb 2020 16:05 |
Status: | Published |
Publisher: | F1000Research |
Identification Number: | 10.12688/f1000research.11652.1 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:156889 |
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