Cosgrove, SC, Mattey, AP, Riese, M et al. (6 more authors) (2019) Biocatalytic Oxidation in Continuous Flow for the Generation of Carbohydrate Dialdehydes. ACS Catalysis, 9 (12). pp. 11658-11662. ISSN 2155-5435
Abstract
Galactose Oxidase (GOase) has been used for the scalable and selective C-6′ oxidation of lactose, a waste material from the dairy industry. Generation of the 6′-oxo lactose was achieved with full conversion in batch mode at milligram scale, but further scale-up to gram quantities proved to be challenging because of requirements for high enzyme concentrations and limitation in oxygen cosubstrate availability. To overcome these issues, a continuous-flow system was developed for the bio-oxidation of lactose yielding multigram quantities of product. Using the variant GOase F2, terminal selective oxidations were also observed on a range of oligoglucosides such as maltose. The carbohydrate dialdehydes that were obtained by this highly selective oxidation were chemically further functionalized, establishing the biooxidation as a route to valorize cheap carbohydrates, including waste materials, for building blocks of polymers.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Catalysis, after peer review and technical editing by the publisher. |
Keywords: | carbohydrate dialdehydes; continuous flow; galactose oxidase; glucosides polymer |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Mechanical Engineering (Leeds) > Institute of Engineering Thermofluids, Surfaces & Interfaces (iETSI) (Leeds) |
Funding Information: | Funder Grant number HEFCE (Higher Education Funding Council for England) no ext ref |
Depositing User: | Symplectic Publications |
Date Deposited: | 17 Dec 2019 12:09 |
Last Modified: | 13 Apr 2022 14:25 |
Status: | Published |
Publisher: | ACS Publications |
Identification Number: | 10.1021/acscatal.9b04819 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:154698 |