Chapman, MR, Cosgrove, SC, Turner, NJ et al. (2 more authors) (2018) Highly Productive Oxidative Biocatalysis in Continuous Flow by Enhancing the Aqueous Equilibrium Solubility of Oxygen. Angewandte Chemie, 130 (33). pp. 10695-10699. ISSN 0044-8249
Abstract
We report a simple, mild, and synthetically clean approach to accelerate the rate of enzymatic oxidation reactions by a factor of up to 100 when compared to conventional batch gas/liquid systems. Biocatalytic decomposition of H2O2 is used to produce a soluble source of O2 directly in reaction media, thereby enabling the concentration of aqueous O2 to be increased beyond equilibrium solubility under safe and practical conditions. To best exploit this method, a novel flow reactor was developed to maximize productivity (g product L−1 h−1). This scalable benchtop method provides a distinct advantage over conventional bio‐oxidation in that no pressurized gas or specialist equipment is employed. The method is general across different oxidase enzymes and compatible with a variety of functional groups. These results culminate in record space‐time yields for bio‐oxidation.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2018 Wiley‐VCH Verlag GmbH & Co. KGaA, Weinheim. This is the peer reviewed version of the following article: Chapman, MR, Cosgrove, SC, Turner, NJ et al. (2 more authors) (2018) Highly Productive Oxidative Biocatalysis in Continuous Flow by Enhancing the Aqueous Equilibrium Solubility of Oxygen. Angewandte Chemie, 130 (33). pp. 10695-10699. ISSN 0044-8249, which has been published in final form at https://doi.org/10.1002/ange.201803675. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. |
Keywords: | Biokatalyse; Flussreaktoren; Kontinuierlicher Fluss; Oxidationen; Raumzeitausbeuten |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Mechanical Engineering (Leeds) > Institute of Engineering Thermofluids, Surfaces & Interfaces (iETSI) (Leeds) The University of Leeds > Faculty of Engineering & Physical Sciences (Leeds) > School of Chemistry (Leeds) > Organic Chemistry (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 08 Nov 2019 10:15 |
Last Modified: | 08 Nov 2019 10:24 |
Status: | Published |
Publisher: | Wiley |
Identification Number: | 10.1002/ange.201803675 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:153125 |