Lyall, GK orcid.org/0000-0002-5986-4845, Davies, MJ, Ferguson, C orcid.org/0000-0001-5235-1505 et al. (2 more authors) (2019) In-exercise vascular shear rate during acute continuous and interval exercise: impact on endothelial function and miR-21. Journal of Applied Physiology, 127 (6). pp. 1754-1762. ISSN 8750-7587
Abstract
Background: Endothelial cell phenotype and endothelial function are regulated by hemodynamic forces, particularly wall shear stress (WSS). During a single bout of exercise, the specific exercise protocol can affect in-exercise WSS patterns, and consequently endothelial function. MicroRNAs might provide a biomarker of in-exercise WSS pattern, to indicate whether a specific exercise bout will have a positive effect on endothelial function. We evaluated the effect of acute interval (IT) and continuous (CON) in-exercise WSS patterns upon post-exercise endothelial function and circulating miR-21 expression. Methods and Results: 13 participants performed CON and 3 different IT exercise protocols matched for duration and intensity, on separate days. Oxygen uptake, heart rate and brachial artery blood flow were recorded throughout exercise. Brachial artery flow mediated dilation (FMD) was performed pre and 15 min post exercise. Plasma samples were acquired pre and 6 hours post exercise to determine miR-21 expression. In-exercise shear-rate (SR) patterns (a surrogate of WSS) differed according to the CON or IT work rate profile. In-exercise anterograde SR was greater in CON than IT (P<0.05), retrograde SR was equivalent between exercise protocols (P>0.05). Oscillatory shear index was higher during IT versus CON (P<0.05). Post-exercise FMD increased (pre 7.08±2.95, post 10.54±4.24%, P<0.05), whilst miR-21 expression was unchanged (pre 12.0±20.7 %cel-miR-39, post 11.1±19.3 %cel-miR-39, P>0.05); with no effect of exercise protocol (P>0.05). Conclusions: CON and IT exercise induced different SR patterns, but equivalent improvements in acute endothelial function. The absence of change in miR-21 expression suggests miR-21 is not a suitable biomarker of exercise-induced SR.
Metadata
Item Type: | Article |
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Authors/Creators: |
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Copyright, Publisher and Additional Information: | © 2019, Journal of Applied Physiology. This is an author produced version of a paper published in Journal of Applied Physiology. Uploaded in accordance with the publisher's self-archiving policy. |
Keywords: | interval exercise; endothelial function; FMD; microRNA; shear rate |
Dates: |
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Institution: | The University of Leeds |
Academic Units: | The University of Leeds > Faculty of Biological Sciences (Leeds) > School of Biomedical Sciences (Leeds) The University of Leeds > Faculty of Medicine and Health (Leeds) > School of Medicine (Leeds) > Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM) > Specialist Science Education Dept (Leeds) |
Depositing User: | Symplectic Publications |
Date Deposited: | 05 Nov 2019 13:54 |
Last Modified: | 10 Oct 2020 00:39 |
Status: | Published |
Publisher: | American Physiological Society |
Identification Number: | 10.1152/japplphysiol.00156.2019 |
Open Archives Initiative ID (OAI ID): | oai:eprints.whiterose.ac.uk:152592 |